Entries by Jon Daniels

Crowd-funding a high-NA multi-immersion objective lens

We have designed a promising high-NA multi-immersion all-immersion objective lens that has applications for both cleared tissue imaging and live-cell imaging.  We would love to make this objective generally available, but we need the help of the research community. The initial design was completed just prior to the COVID pandemic and there are interested researchers, […]

Help us make King Snout a reality

We have received a number of inquiries about the AMS-AGY v2 “King Snout” objective.  We would love to make it generally available, but we need the help of the research community. This objective has been stuck in a chicken-and-egg situation.  ASI needs some orders to feel confident proceeding with a production run.  (If we don’t […]

Preliminary plans for 3rd multi-immersion objective lens

We are going public with tentative plans for our third multi-immersion objective lens. By end-2019 we plan to finalize the design based on feedback, plus decide how large of an order to place for them (the larger the order the smaller the cost, but we have to be assured they will sell). So please get […]

Our second objective lens is here

Our popular multi-immersion objective lens now has a much-anticipated sibling, another multi-immersion lens with increased NA of 0.7.  It is available for immediate sale. Here are some glamour shots:   Like the original NA 0.4 lens, this objective lens is targeted at light sheet imaging of cleared tissue but is useful for a myriad of […]

Does “n” belong? (or, getting light sheet equations correct)

A few months ago I stumbled across a discrepancy between formulas used to describe the imaging and illumination paths of light sheet microscopes.  I believe that one of the oft-cited equations in the world of light sheet microscopy is missing a term.  I could be wrong, but hear me out.  Comments or corrections welcome. The […]

Recent cleared tissue light sheet pre-prints with ASI contributions

Two pre-prints with light sheet microscopes with ASI contributions have been recently submitted to bioRxiv.  Eventually these should appear in traditional journals, but I applaud the authors’ decision to make them available on bioRxiv sooner (I’m a big fan of pre-prints for multiple reasons). First, a pre-print from the Allen Institute for Brain Science describing […]