Oblique Single Plane Illumination Microscope (oSPIM)
About the Oblique Single Plane Illumination Microscope (oSPIM)
ASI’s oSPIM is an excellent platform for imaging live cells or other samples on a coverslip using fast and gentle light sheet microscopy. The oSPIM is a single-view light sheet system where the light sheet is generated at an oblique angle using an oil immersion objective below the sample dish. Fluorescent emission is observed using a high NA water dipping objective from the top, with the objective tilted 60 degrees, perpendicular to the illumination sheet. The arrangement with high NA objectives both above and below the sample dish allow for high-resolution imaging in a convenient geometry for cell culture work.
The oSPIM is two microscopes in one. The lower microscope can be used for fluorescent imaging using conventional modalities, such as wide field fluorescence, confocal, or TIRF. It is also utilized as the light sheet excitation objective. The tilted top microscope is dedicated to light sheet images.
- Low photobleaching: >10x reduction vs. confocal/spinning disk
- Water dipping emission objective yields 280 nm transverse resolution (NA up to 1.1)
- Rapid 3D imaging at oblique angle to coverslip, up to 200 planes per second
- Conventional sample mounting in cell culture dishes
- Fully functional “conventional” fluorescent microscope in addition to the light sheet modality
- Modular and flexible