CRISP Autofocus System
About the CRISP System
The CRISP system is designed to maintain focus over time, i.e. compensate for thermal and other factors that may cause the sample to drift. It also can be used to maintain a given focal point while scanning the sample in XY. If you are looking to find the optimal focal point while scanning through the sample in Z please see our video autofocus system.
CRISP is parted with the Dual C-Mount Splitter (DCMS), camera and tube lenses as part of a microscope. The Dual C-Mount Splitter (DCMS) provides two parfocal C-Mount ports when mounted on many common microscopes. Typical combines a fast analog autofocus camera system simultaneously with a high-resolution digital camera.
CRISP will not work with index matching mounting media. This is how most prepared slides are made. There is no interface with a refractive change near the sample to reflect the light. CRISP relies on reflected light from the sample to detect focus position. Often the reflected light comes from small refractive index discontinuities at sample surfaces. The amount of light reflected at a dielectric interface is given by: R=(n1-n2)2/(n1+n2)2
Where n1 and n2 are the reflective indexes of the adjoining dielectric materials. There must be a difference between the refractive indexes in order for CRISP to work.
CRISP System Details
- Mounts onto any microscope’s standard C-Mount port
- Maintains ideal focus for days
- Works with most normal microscope objectives
- Low noise electronics allows locking to glass/water interfaces
- Integrates with ASI Piezo-Z or motorized focus stages
- Simple post-lock fine adjustment of focus
- Automated control