The CRISP system is designed to maintain focus over time i.e. compensate for thermal & other factors that may cause the sample to drift out of focus over time. It also can be used to maintain a given focal point while scanning the sample in XY. If you are looking to find the optimal focal point while scanning through the sample in Z please see our video autofocus system.
The CRISP system projects the image of a mask illuminated with an IR LED into the sample plane. Only one half of the objective pupil is illuminated. This means that the point spread function of the objective is highly skewed, so that the reflected image of the mask will move laterally as focus is changed. CRISP detects this lateral motion of the LED image to obtain a focus error that is used to close the focus positioning loop.
CRISP is usually installed with ASI Dual C-Mount Splitter (DCMS) that contains the required dichroic beam combiner and blocking filters and provides the C-mount port for the camera.
|Typical Focus Accuracy||<5% of objective Depth of Focus|
|Controller||MS2000 w/ CRISP card|
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