One of the unique needs of video microscopy when using fluorescent labeled images is the ability to precisely limit exposure times. ASI's Shutter Controllers provide both the precise control and the fast response times that are required when working with these dyes.
The SC-2000 is a two-channel shutter controller that provides several means for control of the shutters. Shutter can be activated from a front panel switch, from TTL signals, from foot switches, or via RS-232 serial computer control. In addition to simple on/off control, the controller can also be configured to provide a programmable fixed shutter exposure time. The unit is designed to work with either normally open or normally closed shutters in both the tungsten and ultraviolet light paths of the microscope. Additionally, ASI offers shutters and custom shutter housings for most microscopes.
SC-2000 Shutter Controller - Back View
The switches on the front panel of the SC-2000 controller allow the user to directly activate the shutters. Connections on the back panel provide for serial command, foot switch, and TTL Trigger inputs for each shutter. Additionally, SYNC OUT signals indicate when the shutters are open (if used with shutters equipped with sync sensors). The controller’s firmware is updatable via its serial port using a PC computer by the user.
Specifications
| Number of shutter channels |
Two |
| Activation Time |
Opening a 25 mm N. C. shutter |
8 ms |
| Closing a 25 mm N. O. shutter |
7 ms |
| Peak Unsustained Repetition Rate |
40 Hz |
| Maximum Sustained Repetition Rate |
5 Hz |
| Current Firmware Version |
V1.1 |
SC-2000 Shutter Timing
The following diagram illustrates timing of pulse input and synchronization output relative to a Normally-Closed shutter's state, with labels used in the chart below.
The following chart lists typical timing values (in milliseconds) using 25 mm aperture shutters equipped with standard black Teflon® coated shutter blades (VS25S1ZM1), measured when controlled by an ASI SC-2000 shutter controller.
| Period |
Description |
Normally Open Shutter |
Normally Closed Shutter |
| O-A: |
Delay time on activation after current is applied |
3 ms |
4 ms |
| A-C: |
Transit time on activation |
4 ms |
4 ms |
| O-C: |
Total activation time |
7 ms |
8 ms |
| C-E: |
Minimum dwell time with minimum input pulse |
8 ms |
4 ms |
| B-F: |
Minimum equivalent activation time (50% point) |
13 ms |
5 ms |
| E-G: |
Transit time on deactivation |
2 ms |
6 ms |
| A-G: |
Total window time |
14 ms |
14 ms |
| MET: |
Minimum Exposure Time |
7 ms |
| TEP: |
Typical Exposure Pulse |
> 8 ms |