Tunable lenses are commonly used in light sheet microscopy to adjust the axial position of the beam waist. In addition to being useful for system alignment and automated adjustments, some light sheet acquisition schemes translate the beam waist synchronously with the camera’s rolling shutter to improve axial resolution (e.g. ASLM, Axially Swept Light Sheet Microscopy). The straightforward way to translate the beam waist is by adding a tunable lens at the entrance to the light sheet generator. ASI offers all the necessary hardware to implement ASLM, including both the tunable lens and light sheet generator.
Another application combines two tunable lenses to make an electronically-adjustable beam expander. A final use is to implement an electronically adjustable collimator for white light lasers, compensating for the fiber’s wavelength-dependent divergence point.
The lens is made of an optic-quality deformable polymer manufactured by Optotune. By applying an electric current, the shape of the polymer changes. Thus, the focal length of the lens can be adjusted to a desired value within milliseconds. ASI-designed drive electronics have better performance than alternatives, and also allow tunable lenses to be easily synchronized with other microscope components.
Response time and tuning range depend on the exact tunable lens (and polymer) used; stiffer lenses are faster but have less tuning range. The standard Optotune tunable lens used by ASI has a 12 diopter tuning range and <15 ms transient response. Used with ASI’s 4f relay, it produces 80 µm focus change with a 20x objective and 8 µm of focus change with a 60x objective. The tunable lens can be easily paired with an offset lens to adjust the center of the tuning range.