Dual Selective Plane Illumination Microscopy for Cleared Tissue (ct-dSPIM)

About the Dual Selective Plane Illumination Microscopy for Cleared Tissue

The ct-dSPIM is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (SPIM) that allows for dual views (d) of large samples such as cleared tissue (ct).

The ct-dSPIM is one of many light sheet microscope configurations possible using ASI’s modular components. We manufacture the optomechanical elements, including the motorized stages and 2D galvos for creating and moving the light sheet. ASI partnered with Special Optics to develop an objective optimized for light sheet imaging of cleared tissue. Lasers and sCMOS cameras are required to complete the system; users can procure these themselves, use the services of various system integrators selling ASI SPIM systems, or purchase them via ASI.

The ct-dSPIM has been successfully used to image various cleared tissue samples including whole mouse brains and slices of cleared tissue.

Basic System Configurations

Single-Sided System (iSPIM)

Light sheet created from one objective and imaged using the other objective.

Advantages: Fastest acquisition, least expensive.

Disadvantages: Better XY resolution than Z resolution.

More Info

ct-dSPIM Features
  • Image acquisition >10^8 voxels/sec
  • Sub-micron resolution in XYZ (sample-permitting)
  • Sample mounting in open dish
  • Image >5 mm deep into flat samples or up to 12 mm radius sphere
  • Media RI range from 1.33 to 1.56, aqueous or organic media
  • Modular and flexible setup