Cleared Tissue Objective

Cleared Tissue Objective

ASI and Special Optics have developed an immersion objective lens specifically designed for light sheet microscopy of cleared tissue samples, including ASI’s dual-view Selective Plane Illumination Microscopy (dSPIM), which enables isotropic resolution without manipulating the sample. The lens is also appealing to researchers building customized microscope setups (e.g. OpenSPIM type) because it is available for separate purchase unlike other objectives suitable for cleared tissue.

The 0.4 N.A. multi-immersion objective is designed for dipping media RI ranging from 1.33 to 1.56, >1 mm field of view, ~17x magnification, and 12 mm working distance. When used in multi-view systems like the diSPIM, the cleared tissue objective offers sub-micron resolution in X, Y, and Z.

The large working distance enables imaging samples that were previously inaccessible. The objective permits imaging more than 5 mm deep into a flat sample when rotated at a 45° angle above the sample, as used in the diSPIM.

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Cleared Tissue Objective Features
  • Dual-view cleared tissue imaging (diSPIM with cleared tissues)
  • Single-view cleared tissue imaging (OpenSPIM setups for cleared tissues)
  • Water-dipping applications with very long working distances