Oblique Single Plane Illumination Microscope (oSPIM)
About the Oblique Single Plane Illumination Microscope (oSPIM)
ASI’s oSPIM microscope is an excellent platform for high resolution light sheet microscopy for samples mounted in standard coverslip-bottom culture dishes. The oSPIM is a single-view light sheet system where the illumination light sheet is generated at an oblique angle using an oil immersion objective below the sample dish. Fluorescent emission is observed using a high NA water dipping objective from the top side where this objective is tilted 60 degrees, perpendicular to the illumination sheet. The arrangement with high NA objectives both above and below the sample dish allow for high-resolution imaging in a convenient geometry for cell culture work.
The oSPIM is two microscopes in one. The lower microscope can be used for fluorescent imaging using conventional modalities, such as wide field fluorescence, confocal, or TIRF. It also is utilized as the light sheet excitation objective. The tilted top microscope is dedicated to Selective Plane Illumination Microscopy (SPIM) or light sheet images.