Cleared Tissue Objective

Cleared Tissue Objective

Special Optics and Applied Scientific Instrumentation (ASI), a provider of complex life science and light microscopy solutions, together have developed an immersion objective lens specifically designed for light sheet microscopy of cleared tissue samples, including ASI’s dual-view Selective Plane Illumination Microscopy (diSPIM) which enables isotropic resolution without manipulating the sample. The lens is also appealing to researchers building customized microscope setups (e.g. OpenSPIM type) because it is available for separate purchase, unlike other objectives suitable for cleared tissue.

The 0.4 N.A. multi-immersion objective is designed for dipping media RI ranging from 1.33 to 1.56, has NA ~0.4, >1 mm field of view, ~17x magnification, and 12 mm working distance.  When used in multi-view systems like the diSPIM, the Special Optics objective lens offers sub-micron resolution in X, Y, and Z.

The large working distance enables imaging samples that were previously inaccessible. The objective permits imaging more than 5 mm deep into a flat sample when rotated at a 45° angle above the sample, as used in the diSPIM.

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Applications
Cleared Tissue Objective Features
  • Dual-view cleared tissue imaging (diSPIM with cleared tissues)
  • Single-view cleared tissue imaging (OpenSPIM setups for cleared tissues)
  • Water-dipping applications with very long working distances
Specifications
Cleared Tissue Objective Specifications
Specifications Value Comments
Numerical Aperture 0.4 @ RI 1.45 0.37 – 0.43 over RI range
Immersion Media RI 1.33 – 1.56 Includes all major clearing solutions
Effective Focal Length 12 mm @ RI 1.45 15.3x – 17.9x over RI range w/ 200 mm TL
Working Distance 12 mm (for all RI) 5.1 mm imaging depth with flat sample, 12 mm Ø sphere
Field of View 1.2 mm Ø
Spherical Correction 480 – 1000 nm Diffraction-limited for most  media and λ
Chromatic Correction 480 – 720 nm Performance varies by media, optimized for CLARITY and TDE
Correction Collar None Designed for immersion w/o coverslip
Form Factor Nikon style 61.6 mm parfocal distance, M25 threads, 40 mm OD
Datasheets
Cleared Tissue Objective Datasheet

Cleared Tissue Objective

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