http://www.asiimaging.com/docs/ 2026-05-07T13:42:48+00:00 http://www.asiimaging.com/docs/ http://www.asiimaging.com/docs/_media/favicon.ico text/html 2021-09-23T17:15:12+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adding_rs232_to_tiger?rev=1632431712&do=diff Adding RS232 Connector to Tiger Controller Tiger controllers have a physical USB interface but the connection is an emulated RS232 port (i.e. it will show up as a COM port in Windows via a SiliconLabs IC). See our page on RS232 communication. This technote will guide you on how to install a hardwired RS232 connector to Tiger Controller. text/html 2023-03-25T08:57:49+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adding_shutter_to_filter_wheel?rev=1679749069&do=diff Adding a shutter to a ASI Filter Wheel Be sure the power is off and the Filter wheel is removed from the Microscope and its cables are disconnected. You will also need a Phillips screwdriver, and an ASI cutoff shutter with its installation kit that includes 4 small #2 Phillips screws, and lock washers and a cut-corner cover if the shutter was a Normally Closed shutter. text/html 2021-09-23T17:16:01+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adept_swap_in_ms2000?rev=1632431761&do=diff Swapping Adept Piezo Boards in MS-2000 and RM-2000 controllers Tools Needed You will need the following tools to complete the board swap: * a medium Phillips screwdriver * a small Flat screwdriver * a pair of small wire cutters * a pair of needle nose pliers and text/html 2021-09-23T17:16:00+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adept_swap_in_tg1000?rev=1632431760&do=diff How to Swap ADEPT(Piezo Drive cards) in a TG-1000 Controller This tech note contains instruction on how to remove an ADEPT(Piezo Drive) card from a Tiger Controller(TG-1000) and replace it with another. Tools Need * A Medium Phillips(Star) screwdriver text/html 2021-09-23T17:16:00+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adjusting_ls50_crossed_roller_rail_bearing_preload?rev=1632431760&do=diff Adjusting LS50 Crossed Roller Rail Bearing Preload Linear crossed roller bearings may need to be re-tensioned if the stage plates are disassembled or become loose for any reason. The procedure is the same for any stage. The instructions will refer to the parts on the LS-50 stage shown below. The principles apply to all crossed roller stages. text/html 2021-09-23T17:16:00+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/adjusting_te2000_pzm_rack_and_gear?rev=1632431760&do=diff Adjusting the TE-2000 PZM Rack and Gears Be sure the power is off and the Stage is removed from the Microscope. You will also need a Phillips screwdriver, a 2.5 mm hex ball driver, a Caliper, an assortment of short shim stock or thin metal, and a foam pad a big as the stage. text/html 2021-09-23T17:16:00+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/all_serial_commands?rev=1632431760&do=diff Serial Commands Directory Command:AALIGN (AA) Shortcut AA Format AALIGN [axis] = [alignment]… Units 0-99, hardware potentiometer valueShortcut AA Format AALIGN [axis] = [alignment]… Units 0-99, hardware potentiometer valueType Axis-Specific \begin{equation} Cnts = {(6.5536 * 107) \over d} \end{equation}\begin{equation}X → X + r\end{equation}\begin{equation}r = width × \frac{θ}{2π}\end{equation} text/html 2025-08-25T13:54:07+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/array?rev=1756144447&do=diff ARRAY MODULE for ARRAY Scanning This manual section applies to the ARRAY MODULE firmware module. With the ARRAY MODULE included in the firmware, the controller will support functions associated with moving to specific locations in a 2-dimensional XY array of positions. The module includes support for commands to define the size and spacing of the array and the location of the first position in the array. There are also commands to address particular positions and sequence through the array e… text/html 2024-06-28T15:27:54+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/asi_gamepad?rev=1719602874&do=diff ASI Gamepad This page is deprecated; the new home of this documentation is at ASI Gamepad Plugin ASI Gamepad is a Micro-Manager plugin that adds support for an XBox Gamepad. It lets users assign device properties to thumb sticks and Beanshell scripts to button presses. It is part of Micro-Manager 1.4.x builds as of 24-Oct-2018 and can be found in the menu Plugins > Device Control > text/html 2023-08-28T20:25:06+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/asi_xyz_tracker_plugin?rev=1693268706&do=diff ASI XYZ Tracker Note: This is a Micro-Manager 1.4 plugin, as a company we are moving to Micro-Manager 2.0, and the priority of converting this plugin to 2.0 is based on customer interest. Send us an email if you're interested in this plugin. ASI's XYZ Tracker is a text/html 2021-12-08T18:48:04+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/asi?rev=1639007284&do=diff text/html 2026-03-23T18:08:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/asitiger?rev=1774303716&do=diff ASITiger Device Adapter ASITiger is a Micro-Manager device adapter for the TG-1000 controller. The following tables map the device property names that you see in the device property browser in Micro-Manager to the underlying serial command being used by the device adapter. text/html 2021-09-23T17:15:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/assembly_instructions_for_ramm?rev=1632431759&do=diff Assembly Instructions for the RAMM Notice: RAMM systems are usually shipped intact since at least 2015 so these instructions are obsolete and rarely needed. Be sure to locate and remove all parts and tools from the packaging. Part 1: Parts list Step 1: Remove all the parts from the packaging. They should include: text/html 2021-09-23T17:15:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/atmospheric_spec?rev=1632431758&do=diff Atmospheric Specifications for ASI Tiger Controllers Operating Temperature 10º to 35º celsiusHumidity 0-75% RH non condensingAtmospheric Pressure 700 HPa to 1060 HPa Storage Temperature -20º to 45º celsius †Humidity 10-90% RH non condensing text/html 2021-09-23T17:15:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/autofocus_standalone?rev=1632431758&do=diff Video Auto-Focus Card: Standalone Operation Manual Introduction The ASI auto-focus system is an electronic circuit that detects the high spatial frequency information present in a video signal coming from an analog camera, and converts it to a focus signal text/html 2021-09-23T17:15:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/autofocus_termination?rev=1632431758&do=diff Proper Termination for ASIs Autofocus Card The Input Video IN of the Autofocus Card is terminated with a 10KΩ resister, as it expected to share the video signal coming from the camera with other devices like a monitor or video capture card, which usually have 50Ω or 75Ω termination in them. text/html 2021-09-23T17:15:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/av200_flattop_installation?rev=1632431758&do=diff AV200 Flat Top Stage Installation Remove System from packaging. Remove the stage locking bolt and connect stage controller with the provided cable. Connect the power brick to controller and turn on the system. Then, place the stage onto microscope stand. text/html 2024-11-26T19:18:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/bpu_operation?rev=1732666696&do=diff ASI BPU Back Pressure Unit Operating Instructions The Back Pressure Unit is designed to work with the MPPI-3 or MPPI-2 Pressure Injector. When properly connected, the Back Pressure Unit provides a regulated back pressure to the injection pipette between pulses. To properly connect the Back Pressure Unit to the MPPI-2 please refer to the diagram in the MPPI manual. text/html 2021-09-23T17:15:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/c60_slider_cover?rev=1632431757&do=diff C-60 Slider Cover Instructions These are the three parts that you should have received from ASI. Start by removing old covers. First cover pulls off by hand, Second cover you will need 2 hex wrenches 2mm and a 2.5mm. Remove screws and save screws for reassembly. text/html 2021-09-23T17:15:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/changing_joypod_header_rm2000?rev=1632431757&do=diff Changing the Joypod header in a RM-2000 Tools Needed: * a medium Phillips screwdriver, * a small Flat screwdriver, * a 3/16” socket driver, * a 16 pin Joypod header and * maybe a pair of wire cutters. First, using the Phillips screw driver, remove the nine lid screws that attach the top of the rack controller, (see picture above). Three are on the left, three on the right and three in the middle, (see pictures below). text/html 2021-09-23T17:15:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/changing_z_linear_encoders_on_ms2000?rev=1632431757&do=diff Changing Z Linear Encoder Headers on the MS-2000 You should be familiar with the MS/MFC-2000 controller before attempting the header swapping, and you will need a 3/16” socket driver and small pliers. STEP 1: Turn off the power and remove all cables attached to the controller. text/html 2021-09-23T17:15:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/cleared_tissue_objective?rev=1632431757&do=diff text/html 2026-02-19T18:11:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/command_quick_start?rev=1771542710&do=diff Quick Start on Serial Commands This section is intended to quickly bring the users up to speed on the most common serial commands. Find separately an index of all commands and a compiled list of commands; separate documentation for each command are linked there. There is a separate page detailing the text/html 2021-09-23T17:15:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/commands?rev=1632431756&do=diff Commands text/html 2021-09-23T17:15:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/comparison_of_asi_closed_loop_stage_and_control_electronics_with_competing_products?rev=1632431756&do=diff Comparison of ASI’s Closed-loop DC Servomotor Stages and Control Electronics with Competing Products ASI's closed-loop DC servomotor stages and control electronics have been designed to provide an extremely precise and highly repeatable platform for demanding microscopy applications. The stages are manufactured by skilled craftsmen in the United States with high quality components under stringent quality control procedures. Due to the quality of the components used, and extensive on-going res… text/html 2021-09-23T17:15:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/conix_ms4_zdrive?rev=1632431756&do=diff Conix MS-4 Z-Axis Drive Installation The open loop belt drive attaches to the coarse focus knob via the thumbscrews as shown in the photos below. The fine focus knob pulley is attached to the fine focus knob as shown via the setscrews located on the fine focus knob pulley. The belt is stretched between the two pulleys as shown and once in place the cover is installed. The drive is connected to the controller via the connector on the rear of the drive. text/html 2021-09-23T17:15:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/connection_differences_madcity_adept?rev=1632431756&do=diff Connection differences for Mad City Piezo to Adept Piezo upgrades When upgrading from a Mad City Piezo control box to an ASI Adept Piezo control box there are several differences to note regarding connections. The DB-9 Male cable from the Piezo Z part of the stage connected to the MCL Nano-Drive at the DB-9 Female will now connect to the text/html 2021-09-23T17:15:55+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/crisp_alignment_oem?rev=1632431755&do=diff Basic CRISP alignment for OEM systems Setup as follows: * Representative test sample in place * Recording camera present with no filters between CRISP dichroic and camera * CRISP cover and light shield removed for access to the LED fastener * text/html 2026-03-08T08:00:12+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/crisp_manual?rev=1772971212&do=diff CRISP : Continuous Autofocus System The Continuous Reflection Interface Sampling and Positioning (CRISP) system provides for a very high level of focus stability, allowing a specimen to remain accurately focused for hours at a time with drift <0.1 μm. The system compensates for focus changes caused by temperature variations as well as mechanical drifts of the microscope mechanisms. The CRISP solves the focus drift problem that plagues time-lapse experiments at high magnification, and also all… text/html 2024-07-22T17:22:57+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/crisp_mm_plugin?rev=1721683377&do=diff ASI CRISP Control For general information about CRISP, please visit the manual. Setup The plugin comes with Micro-Manager 2.0 by default, it also comes with Micro-Manager 1.4 but is not actively developed. First, make sure that you add CRISP to your hardware configuration. You can access the text/html 2024-07-26T15:40:02+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/crisp_ninja?rev=1722022802&do=diff CRISP Ninja (Standalone CRISP GUI) CRISP Ninja is a standalone application that works with CRISP on both the TG-1000 and MS-2000 controllers. This is a Windows only application and works on Windows 10 and 11. The CRISP Micro-Manager plugin can also be used to control CRISP. text/html 2021-09-23T17:15:11+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/customer_assembly_instructions?rev=1632431711&do=diff Assembly Instructions for Customers XY Stages RAMM VTS Modular Microscopes (MIM) Installing a Mirror or D-cube in C60-CUBE There are two set screws that hold the dichroic filter holder onto the dovetail of the cube. One of these is a typical set screw with a green Nylon tip, for tightening the dichroic holder in place securely. The other set screw has an acentric cam protrusion that can move the dicroic vertically. The dichroic holder should be as close to centered as is possible. text/html 2021-09-23T17:15:55+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/dcms_manual?rev=1632431755&do=diff DCMS Instruction Manual The DCMS is a Photo-port Beam Splitter that accepts either Dichroics or Mirrors in the Standard Mirror Plate Dimensions of 36 x 25.5 x 1.1mm, with Filter Dimensions of 25 x 3.7mm (round). The cube disassembles into two halves by the removal of the four 2.5mm retaining cap head screws. And the two C-Mount filter holders are removed or adjusted for rotation by the three 1.5mm cone tip set screws located around each mount. text/html 2021-09-23T17:15:54+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/epi_illumination_options?rev=1632431754&do=diff Epi-Illumination Options and Laser Coupling The flexibility of the light path on the ASI Modular Infinity Microscope (MIM) brings with it the challenge of properly designing epi-illumination so that the desired intensity and uniformity of the illumination light are achieved. We will discuss various fluorescence illumination condenser options and the pros and cons of various approaches.\begin{equation} FOV=d_{Light Guide} \frac{F_{Tube Lens}}{F_{Condenser}} =3mm \frac{200 mm}{25 mm}=24mm \end{… text/html 2026-03-13T14:48:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/error_codes?rev=1773427709&do=diff Error Codes for MS2000 and TG1000 Diagnostics Error codes are dumped to the serial terminal with the last error code shown first using the DU Y command. If you're looking for such as N-1, N-2, etc you can find a table at the bottom of the page. If you're looking for text/html 2025-03-17T16:14:13+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/filter_and_turret_changer?rev=1742242453&do=diff Objective Turret & Slider Operation The MS-2000 and TG-1000 controller supports several clocked devices, such as motorized nosepiece objective turrets, filter cube turrets, and filter wheels. ASI’s motorized turret systems only power the motor when actually making the move to position. The motors are disconnected otherwise, allowing the user to manually move the turret to any position. Gears and encoders remain connected, so the actual turret position is always known by the controller. text/html 2024-04-16T18:47:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/flashing_ms2000_controller?rev=1713307670&do=diff Flashing Firmware ASI controllers ship with a bootloader that allows new firmware provided by ASI to be installed in the field. Firmware will be provided by ASI as needed. The bootloader itself should not be changed. Updating update on the the MS2000 or RM2000 controller is done with the text/html 2021-09-23T17:15:53+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/four_axis_joystick_wheel_ops?rev=1632431753&do=diff Four-Axis Joystick and Wheel Operation Precise manual control of micromanipulators, or other multi-axis instruments is possible with the MS-2000’s fine resolution joystick or command encoder wheel. Depending upon the application or configuration, the user may wish to use the joystick or the command wheel as the manual input device for a particular axis. text/html 2021-09-23T17:15:53+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ftp_controller?rev=1632431753&do=diff FTP Controller The toggle switch near the joystick provides for a rapid means of moving the stage up & down. Fine control of z position is accomplished with the Z focus knob, or by computer control. There is an option to control the Z focus with foot switches as well. If this option is requested the 9 pin serial out connector is used to connect the foot switches, and is labeled as such. text/html 2023-10-11T00:40:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ftp_setup?rev=1696999236&do=diff FTP-2000 Setup & Alignment ftp manual Parts List 1 - Tiger controller and FTP firmware 1 - XY stage 2 - Stilts (single-axis stages with mounting brackets; one master, one slave 1 - Optical breadboard ≥ 600 mm wide 1 - Ruler or measuring tape 1 - 5 mm hex driver 4 - M6 washers 4 - M6 x 25 mm socket head screws (stilts to metric breadboard) text/html 2023-03-26T05:10:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/fw_1000_old?rev=1679821805&do=diff Old FW-1000 Control Box Manual The FW-1000 Control Box has been deprecated, and is no longer available. The Shutter Control Panel shown in the photo below has been deprecated, and is no longer available. The current filter wheel hardware options available are the Standalone FW-1000-SA unit and the TGFW card inside the text/html 2025-02-19T17:53:34+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/fw_1000?rev=1740005614&do=diff FW-1000 Filter Wheel Manual The Filter Wheel Drive Card is available in two configurations, as part of the TG-1000 Tiger Controller or as a Standalone FW-1000-SA unit. Operation is identical except for the save settings command, see RAM Save/RAM Write. Warnings * text/html 2023-03-25T06:05:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/fw1000_and_mm?rev=1679738741&do=diff Micro-Manager 1.4 and Filter Wheel This Technical note is to document the known issue when connecting micro-manager(MM) with ASI's filter wheel and work around for it. On Startup MicroManager does the following: * First MM does is to issue serial command text/html 2021-09-23T17:15:52+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/fw1000_optical_encoder_adjustment?rev=1632431752&do=diff FW-1000 Optical Encoder Adjustment (Be sure to have a copy or the FW-1000 Manual for the final software alignment of the Filter Wheel) Step 1: You will need the following tools to adjust the encoder: a Phillips screwdriver, a 3/32” Allen wrench and a.050” Allen wrench. text/html 2021-09-23T17:15:52+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/gantry_translation_stage?rev=1632431752&do=diff Gantry Translation Stage (GTS-1500) INTRODUCTION This manual outlines the operation of ASI’s GTS (Gantry Translation Stage) control system. The GTS is a large, highly functional, computer-controlled stage system than can be configured in several different ways, depending upon the needs of the user. The basic system consists of the control unit, the GTS X-Y-Z stage. text/html 2021-09-23T17:15:52+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/getting_started_with_dokuwiki?rev=1632431752&do=diff Getting started Creating/Editing Pages in this Wiki At the moment , Creating and editing content on this site is restricted to ASI staff. Please contact ASI if like you like any additions or corrections. Creating a New Page * Begin by typing the Namespace and name of the new page into the Search field and search\begin{equation}R = \frac{(n_1 – n_2)^2}{(n_1 + n_2)^2}\end{equation} text/html 2022-10-25T18:23:54+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/hardware?rev=1666736634&do=diff Hardware This page is a work in progress... It should list all of ASI's available hardware and link to their relevant manuals/info. products hardware text/html 2021-09-23T17:15:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/heidenhain_xy_le?rev=1632431751&do=diff Heidenhain, Renishaw and MicroE XY Linear Encoder Installation Congratulations on your choice of this option. The Tiger and MS2000 with XY linear encoders offers unparalleled accuracy and repeatability. The encoders used on MS2000 stages are optical devices with a count resolution of 10nm(with Heidenhain and Renishaw ) and 2.5nm(MicroE). In order to give this superb performance, care must be taken in the handling of the encoders, scales, and stage. text/html 2023-04-20T17:47:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/i-3020?rev=1682027279&do=diff tech_note I-3020 MLLAC The I-3020 MLLAC Multi well plate auto locking insert utilizes a Solenoid lock with a spring to drive a cam in and out of locking position. The insert is driven by a microprocessor in the controller and a solenoid driver board in the insert. The solenoid only consumes and dissipates power when in the unlock state. Over time the heat generated by this power dissipation may damage the solenoid. text/html 2021-09-23T17:15:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/improve_piezo_reliability?rev=1632431751&do=diff Improving Piezo based Stages/Actuators Lifetime and Reliability This tech note is a collection of tips and best practices when working with ASI's Piezos to maximize their lifetime and reliability. Piezo On Time and Lifetime Most piezo stack manufacturers specify the lifetime in hours at maximum rated voltage. In usage text/html 2026-02-13T15:16:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/infinity_microscope_basics?rev=1771013775&do=diff Infinity Microscope Basics This page is still under serious construction, lots of sections need to be expanded This overview is meant to complement other available resources online such as <https://www.microscopyu.com/microscopy-basics/infinity-optical-systems>. At ASI we make modular microscopes in arbitrary configurations, which has far more flexibility than standard \begin{equation} M = F_{TL} / F_{obj} \end{equation}$M^2$$RI$$RI$\begin{equation} L_{coll} = (⌀_{vig} - ⌀_{BFP}) \times F_{TL… text/html 2021-09-23T17:15:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/inserting_pipette_into_pipette_holder?rev=1632431751&do=diff Inserting Pipette into Pipette Holder 1) Disassemble the O-ring compression subassembly from the pipette support subassembly. 2) Insert the pipette through the support subassembly, and through the O-ring. 3) Lightly tighten the outside nut to clamp the pipette without crushing it. text/html 2021-09-23T17:15:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/install_ftp_nikon?rev=1632431750&do=diff Nikon FN1 Installation Instructions for ASI FTP-2100-XYZLE System The FTP-2100 allows for 100 mm of Z travel when used with the adapter kit shown in figure 1 below. The dual LS-100 units move the complete XY stage in the Z axis and the spacers place the microscope body, trinocular head, focus bar, and objective nose piece in the correct location to allow the full range of travel. The travel range allows for a large number of samples to be used from standard glass slides to whole samples u… text/html 2021-09-23T17:15:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/install_linear_encoder_buffer_card_on_tiger?rev=1632431750&do=diff Installing and Configuring Linear Encoder Buffer Card on Tiger/TG-1000 The following tech note will walk user thru installing, configuring and troubleshooting any issues related to the Linear Encoder Buffer Card. Linear Encoder (LE) buffer card is an add-on card for Two Axis card (configured either as a XY or ZF card) to enable them to interface with either MicroE, Renishaw and Heidenhain brand Linear Encoders. text/html 2021-09-23T17:15:51+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/installing_insert?rev=1632431751&do=diff Installing Inserts Into an ASI XY Stage The easiest way to install the ASI slide insert into the XY stage is to slide the insert in from the right, towards the spring clips, as shown in figures 1 through 3 below. Note: on FTP system , Please insure that power is on to the text/html 2021-09-23T17:15:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/installing_ms4000?rev=1632431750&do=diff Installing the MS-2000 MS-4000 XY Stage onto an Upright Microscope The procedure has three parts: Removing the original stage. Installing the MS-2000 MS-4000 XY stage on the microscope Connecting the cables to the MS-2000 control unit Part 1 – Remove the Original Stage text/html 2021-09-23T17:15:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/joystick_connector_check?rev=1632431750&do=diff Joystick Connector Check If you have a controller with a Joystick , and you get No JS X:0 No JS Y:0 On serial out . Chances are the Joystick connector might have come undone during shipping. You can check if the connector this secure by opening the controller lid. text/html 2021-09-23T17:15:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/joystick_lubrication?rev=1632431750&do=diff Joystick lubrication instructions Use only an approved ASI joystick lubrication, or a silicon based/plastic safe one, on the joystick, other lubricants may soften the plastic parts of the joystick Step 1: Clean the ball hub of the joystick with clean dry air and a cotton swab while moving the stick to its full range of motion in both X and Y. text/html 2023-08-15T15:44:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/julia?rev=1692128698&do=diff Julia You can use the Julia programming language to control ASI hardware. Last tested on Windows 10 64-Bit and Julia 1.8.0 using LibSerialPort # First open the Julia REPL and enter: # using Pkg # Pkg.add("LibSerialPort") # => This will install the LibSerialPort package # get a list of serial ports ports = get_port_list() println("ports = $(ports)") # setup serial port port_name = "COM4" baud_rate = 115200 function serial_send(serial_port::SerialPort, data::String, report::Bool = true) … text/html 2026-04-10T15:28:11+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/labview?rev=1775849291&do=diff LabVIEW ASI has limited official support for LabVIEW which is captured in a set of LabVIEW VIs found at LabVIEW serial support. Behind the scenes everything works based on exchanging serial messages with the ASI controllers. See our Quick Start on Serial Commands page if functionality is required that is not explicitly implemented in the LabVIEW VIs. text/html 2025-05-19T14:57:21+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/lead_screw_pitch_options_xystage?rev=1747681041&do=diff Lead Screw Pitch Options for ASI XY Stages: Speed, Resolution, and Repeatability The following table shows tested specifications for different lead screws pitches, motor gear-head combinations and encoder types. 4 TPI is a good general-purpose choice for for XY stages. 2 TPI is used to boost XY speed. 16 TPI is default for vertical applications and for scan-optimized stages. text/html 2021-09-23T17:15:49+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/legacy?rev=1632431749&do=diff Legacy Documentation Manuals * ADEPT user manual * Advanced Applications * ASI MS2000 SEQUENCER MODULE * ASI TIRF Injector Manual * ASI-PZ-WK-Inverted-XY * Auto-Focus-2-Subdocument * CRIFF Manual * CRISP Autofocus Manual * diSPIM_Manual * Epi-Illumination on the RAMM * FilterWheel Tiger * FW-1000 Manual * FW-1000 Motor Adjustment * Inserting Pipette into Pipette Holder * MicroMirror Drive Card User Guide * MIM & RAMM Manual * MPPI-3 Manual * … text/html 2021-09-23T17:15:49+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_aristoplan_xystage?rev=1632431749&do=diff Installation of ASI MS-2000 Automated XY stage onto Leica Aristoplan Microscope This procedure outlines the steps necessary to install the ASI MS-2000 automated XY stage onto Leica ARISTOPLAN microscope. The procedure has 4 steps: * Removing the condenser carrier. text/html 2021-09-23T17:15:49+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_aristoplan_zdrive?rev=1632431749&do=diff Leica Aristoplan Microscope Motor Drive Installation Motor Drive Installation This procedure steps you through the installation and alignment of the ASI motor drive onto the Leica Aristoplan microscope. The following tools are required for this procedure: text/html 2021-09-23T17:15:49+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dm2000_dm2500_zdrive_install?rev=1632431749&do=diff Leica DM2000 & DM2500 Left-Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Leica DM 2000/2500 microscope To perform the following steps, you will need the following tools: text/html 2021-09-23T17:15:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dm2000_linear_encoder_install?rev=1632431748&do=diff Leica DM2000 Z linear encoder installation instructions The linear encoder stop mounts onto the bottom of the stage as shown in the above photos Step # 2 Installing the encoder clamp & Aligning the encoder Locate the Heidenhain encoder and the encoder clamp. Use the 3/32 Allen wrench to insure that the screw on the side of the encoder clamp is loose. Install the encoder clamp across the rear throat of the microscope as shown. Use the 5/64 inch Allen wrench to tighten the set screw l… text/html 2021-09-23T17:15:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dm3000_zdrive_install?rev=1632431748&do=diff Leica DM3000 Microscope Z-Drive Installation Depending upon your specific order your Z drive may have shipped with a flex coupler that is designed to allow some flexibility between the ASI Z drive & the fine focus shaft of your microscope. The microscope shown in the installation photos shown here is an Olympus IX71, however the installation of the flex coupler is the same for the Leica DMI3000 & all inverted microscopes. The flex coupler mounts to the microscope as shown below, and the text/html 2021-09-23T17:15:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dm4000_dm6000_zdrive?rev=1632431748&do=diff Leica DM4000 to DM6000 Left-Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Leica DM 4000 to DM6000 microscope. To perform the following steps, you will need the following tools: text/html 2021-09-23T17:15:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dmil_zdrive?rev=1632431748&do=diff Leica DMIL Microscope Z-Drive Installation The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Leica DMIL microscope. To perform the following steps you will need the following tools: * Small flat blade screw driver text/html 2021-09-23T17:15:48+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dmirb_zdrive?rev=1632431748&do=diff Leica DMIRB Microscope Z Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Leica DMIRB microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dmlb_zdrive?rev=1632431747&do=diff Leica DMLB Microscope Motor Drive Installation Motor Drive Installation This procedure steps you through the installation and alignment of the motor drive onto the Leica DMLB microscope. The following tools are required for this procedure: * small flat blade screwdriver text/html 2021-09-23T17:15:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_dmrb_xystage?rev=1632431747&do=diff Installation of ASI MS-2000 Automated XY stage onto Leica DMRB Microscope This procedure outlines the steps necessary to install the ASI MS-2000 automated XY stage onto Leica DMRB microscope. The procedure has 4 steps: * Removing the condenser carrier. text/html 2021-09-23T17:15:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_leitz_laborlux_s_linear_encoder_install?rev=1632431747&do=diff Leica / Leitz LABORLUX S Microscope linear encoder installation The procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Leica/Leittz LABORLUX S series microscopes. The linear encoder mounts to the upper front of the microscope’s armature via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip mates with a plunger stop that is attached on the microscope’s car… text/html 2024-11-26T18:44:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_leitz_laborlux_s_zdrive_install?rev=1732664656&do=diff Leica / Leitz LABORLUX S Microscope Motor Drive Installation The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Leitz LABORLUX S microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_mzfl3_zdrive_and_filter_turret_install?rev=1632431746&do=diff Leica Stereo Zoom MZFL III Automated Zoom & Automated Filter Turret Drive Installation Procedure The procedure below outlines the steps necessary to install the Automated Zoom & Filter Turret Drive onto the Leica Stereo Zoom microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leica_stereo_mzfl_3_zdrive?rev=1632431746&do=diff Leica Stereo Zoom MZFL III Automated Zoom & Automated Filter Turret Drive Installation Procedure The procedure below outlines the steps necessary to install the Automated Zoom & Filter Turret Drive onto the Leica Stereo Zoom microscope. == To perform the following steps you will need the following tools: == text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_diaplan_zdrive?rev=1632431746&do=diff Leitz Diaplan Microscope Motor Drive Installation This procedure steps you through the installation and alignment of the ASI motor drive onto the Leitz Aristoplan or Diaplan microscope. The following tools are required for this procedure: * small Phillips screwdriver text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_dmrb_linear_encoder_install?rev=1632431746&do=diff Leitz DMRB microscope linear encoder Installation The procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Leitz DMRB microscope. The linear encoder mounts to the rear of the microscope via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip mates with a plunger stop that is attached to the microscopes stage carrier. The linear encoder installation has two part… text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_dmrb_zdrive?rev=1632431746&do=diff Leitz DMRB Microscope Motor Drive Installation This procedure steps you through the installation and alignment of the ASI Z-axis motor drive onto the Leitz DMRB microscope. The ASI drive is attached to the right hand side of the microscope as shown in figure # 1. The drive is securely attached to the fine focus shaft of the microscope via a “metal on metal clamp”, as shown in figure # 5, which insures a solid and reliable connection. The drive is attached to the microscope’s body via two meta… text/html 2021-09-23T17:15:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_laborluss_zdrive?rev=1632431746&do=diff [OLD]Leitz LaborLux S Microscope Motor Drive Installation This procedure steps you through the installation and alignment of the DRV-Lablux-S motor drive onto the Laborlux-S microscope. The following tools are required for this procedure: * small Phillips screwdriver text/html 2021-09-23T17:15:45+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_laborlux_xystage?rev=1632431745&do=diff Leitz LaborLux D Microscope XY Stage Installation This procedure steps you through the installation and alignment of the MC-XYZ xy stage onto the Laborlux-D microscope. The following tools are required for this procedure: small slotted screwdriver 1.5mm hex wrench (provided) 3mm hex wrench (provided) text/html 2021-09-23T17:15:45+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/leitz_orthoplan_zdrive?rev=1632431745&do=diff Leitz Orthoplan Microscope Motor Drive Installation This procedure steps you through the installation and alignment of the ASI Z axis motor drive onto the Leitz Orthoplan microscope. The following tools are required for this procedure: * Small slotted screwdriver text/html 2025-05-22T15:45:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/low_level_command?rev=1747943129&do=diff Low Level Command Set SETUP CONTROL COMMANDS Currently, the only way to access the low level format is through the Setup Control Commands. The following are special commands used to setup different properties of the MS-2000 and MFC-2000. The MS-2000 and the MFC-2000 recognizes these two byte commands by their prefix byte 255. These commands mimic the Ludl Interface Control Commands and expand upon them. text/html 2024-11-26T18:26:03+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ls50?rev=1732663563&do=diff LS-50 and other Linear Stages This manual pertains to ASI’s LS-Series linear positioning stages used with the MS2000 or Tiger Multi-card controller systems. This manual will describe the installation, operation, and programming for basic system components, plus sections for applicable options. Please contact text/html 2025-03-17T16:08:34+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/lx2_standalone?rev=1742242114&do=diff LX2 Standalone Operation ASI's LX2 card is capable of driving two motorized axes and is available with a special build of MS2000 firmware. This allow the LX2 card to be operated in standalone configuration without any additional electronics to support it. LX2 supports the same serial commands as MS2000 controller. The LX1 is identical in terms of interfacing, but supports only a single motorized axis. text/html 2024-04-18T11:54:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/matlab?rev=1713455687&do=diff MATLAB ASI doesn't have any official support for MATLAB. However you can use MATLAB by sending serial commands to ASI controllers. See our Quick Start on Serial Commands page. Here is a unofficial script to control ASI stages using an MS2000: <https://www.mathworks.com/matlabcentral/fileexchange/19428-asi-microscope-stage-mover>. ASI officially supports Micro-Manager which can be accessed via Matlab. Micro-Manager provides a uniform text/html 2022-02-25T11:28:24+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/micro-manager?rev=1645806504&do=diff Micro-manager Micro-Manager is a free open-source software program that facilitates use of microscope hardware. ASI hardware is well-supported in Micro-Manager via device adapters (part of the MMCore module), which can in turn be accessed via Python, Matlab, or other software programs. text/html 2021-09-23T17:15:44+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/microladd_zdrive?rev=1632431744&do=diff Micro Ladd Technology Z-Drive Installation Procedure You will need * 1.5, 2, 3mm Hex Wrench * Needle Nose Pliers Install Brass Adapter You will need to remove the left fine focus knob by holding the course and fine focus knobs on the right and unscrewing the left hand fine focus knobs. text/html 2021-09-23T17:15:44+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/micromirror?rev=1632431744&do=diff MicroMirror Drive Card User Guide The MicroMirror Drive card is a module for the TG-1000 system. Using this card user can control X and Y axes of 2 MEMS MicroMirrors. The card can be operated in external input mode (0 to 5V analog signal) or in internal input mode (thru onboard DAC so the mirror can be positioned thru serial commands and built in routines). \begin{equation} \frac{20V}{131V}×9.2º = 1.4º\end{equation} text/html 2026-04-28T19:29:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mim_ramm_vts?rev=1777418976&do=diff MIM, RAMM and VTS-2100 Systems Instruction Manual Modular Infinity Microscope (MIM) Rapid Automated Modular Mounting(RAMM) Video Test Stand (VTS-2100)   Introduction The MIM / RAMM /VTS systems consist of a number of modular components that allow rapid assembly of automated optical microscope systems. The Modular Infinity Microscope (MIM) consists of several optics sections, couplers, objective focus devices, beam splitter modules, light sources, etc. that can be constructed into a high perf… text/html 2024-11-26T19:38:54+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mimph_mpip_kit?rev=1732667934&do=diff MIMPH-MPIP Kit Microinjector Pipette Holder The Microinjector Pipette Holder is designed to hold glass drawn micropipettes in a stable, easy to mount, easy to clean, autoclaveable, stainless steel body. MIMPH-MPIP Kit - PDF Manual The MIMPH-MPIP Kit includes: text/html 2021-09-23T17:15:43+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mintron_hdmi_camera_startup_settings?rev=1632431743&do=diff Mintron HDMI Color Camera Startup Settings The Mintron HDMI camera is initially setup in an automatic mode that decides to use either the Color or Black & White mode based on how much light is available every time it is turned on. However the user may want to use only color at the startup. These instructions will guide you through the steps to change those settings. text/html 2024-11-26T18:45:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mippi_kit?rev=1732664759&do=diff MPPI Kit This is the old version of the kit, the documentation for the new MPPI kit can be found here MIMPH-MPIP Kit. deprecated mppi manual text/html 2026-03-06T16:43:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/modules?rev=1772833436&do=diff Firmware Modules You can query the firmware modules with the BU X command. Please contact ASI if you need access to a module that you don't currently have. The # means that the value is a number, for example you could have either RING BUFFER 50 or RING BUFFER 250 text/html 2021-09-23T17:15:43+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mounting_fw1000_on_ramm?rev=1632431743&do=diff Mounting FW-1000s on RAMMs for large size cameras Tools Needed: You will need a 1.5mm and 2.0mm Allen drivers to mount this Filter Wheel. When using a ASI Filter Wheel with a ASI Rapid Automated Modular Microscope, (RAMM), you may find that some of the larger format cameras will not fit within the width of the Filter wheels C-Mount to motor housing or will not rotate fully on the C-Mount to clear the motor housing. To alieviate this problem the Filter Wheel can be mounted backwards on the RAM… text/html 2024-11-25T14:26:56+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/mppi3?rev=1732562816&do=diff MPPI-3 Pressure Injector Summary of Operation The Milli-Pulse Pressure Injector, Model MPPI-3, is a self-contained device for producing gas pressure pulses to an injection pipette. The unit offers linear control of both pressure and pulse duration. text/html 2021-09-23T17:15:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms8k_insert?rev=1632431741&do=diff Installing the Insert in the ASI MS-8000 Stage Attention should be given to the secure installation of the insert into an MS-8000 stage to prevent its movement during operation. Any accidental shifting of the insert during movement will adversely affect the stage’s repeatability properties. text/html 2021-09-23T17:15:42+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms2000_cable_connections?rev=1632431742&do=diff Detailed Pictorial MS-2000 Cable Connection Instructions This procedure gives pictorial instructions on how to connect nearly all of the cables to your MS-2000 controller. (The USB and Serial-Out cables are not covered here.) Please note that, depending upon what options you have selected when purchasing your unit, some of the connections mentioned may not be available on the unit that you received. text/html 2025-10-22T13:46:34+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms2000_operation?rev=1761155194&do=diff MS2000, MFC2000, and RM2000 OPERATION Front Panel Controls XY Joystick Note: MFC2000 controllers do not have a joystick. The XY Joystick is spring loaded to return to a zero movement center when not in use. The speed at which the stage moves is linear function of the degree to which the joystick is pushed away from the center. The direction of deflection can be controlled by the settings of the DIP switches on the back panel of the box (see text/html 2024-08-30T17:24:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms2000_optimal_alignment_procedures?rev=1725053045&do=diff MS-2000 Controller Optimal Alignment Procedures This page is largely obsolete, especially details about setting AA which is set at the factory and does not normally need to be changed. For obtaining fastest possible move times see the more up-to-date text/html 2021-09-23T17:15:42+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms2000_stage_install?rev=1632431742&do=diff Installing the MS-2000 XY Stage onto your Inverted Microscope The procedure has three parts: Removing the original stage. Install the MS-2000 XY stage on the microscope Connecting the cables on the MS-2000 control unit Part 1 – Remove the Original Stage text/html 2021-09-23T17:15:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ms8000_intro?rev=1632431741&do=diff MS-2000 and MS-8000 stage This manual pertains to ASI’s MS-2000 and MS-8000 stage system. The MS-8000 is a 8” (200 mm). Both stages are compact, highly functional, computer-controlled stage system that can be configured in several different ways, depending upon the needs of the user. The basic system consists of the MS 2000 control unit and an MS-8000 XY stage. ASI’s precision Z-axis focus controller can be incorporated to create an integrated XYZ system. For ultimate accuracy and repeatabi… text/html 2026-04-08T15:44:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/multi-immersion_objectives?rev=1775677466&do=diff Multi-Immersion Objectives Summary specifications (click link to jump to details) 57-12-19 54-10-12 54-12-8 57-12-5 Nominal Numerical Aperture 0.3 0.4 0.7 1.2 Approximate Magnification 10x 17x 24x 40x Working distance [mm] 18.1 12.1 10.0 3.3 Nominal Refractive Index 1.53 1.45 text/html 2025-08-27T09:57:23+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/multiaxis?rev=1756303043&do=diff Multi-axis function The multi-axis firmware module lets the controller generate patterns like a circle and spiral. It is implemented but has not yet undergone extensive testing. It can also be used for XY stages if the firmware module is present. tiger ms2000 advanced_feature text/html 2021-09-23T17:15:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_80i_left_zdrive_install?rev=1632431741&do=diff Nikon 80i and 90i Left-Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon 80i microscope. To perform the following steps, you will need the following tools: text/html 2021-09-23T17:15:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_80i_linear_encoder_install?rev=1632431741&do=diff Nikon 80i and 90i Z-axis linear encoder installation The linear encoder holder goes over the linear encoder stop. The hole in the holder should be centered over the metal stop plate. The linear encoder holder mounts onto the microscope body using the two existing tapped holes shown. Remove the two original screws and use the screws supplied to mount the linear encoder holder. As shown below text/html 2021-09-23T17:15:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_az100_zdrive_install?rev=1632431741&do=diff Nikon AZ100 Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon AZ100 microscope. To perform the following steps you will need the following tools: * Medium flat blade screwdriver text/html 2021-09-23T17:15:40+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_diaphot_200_300_te200_te300_zdrive_install?rev=1632431740&do=diff Nikon Diaphot 200/300 & TE200 /300 Z-Axis Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Diaphot microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:40+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_diaphot_tmd_zdrive_install?rev=1632431740&do=diff Nikon Diaphot TMD Microscope Z Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Diaphot TMD microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:39+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_600_zdrive?rev=1632431739&do=diff Nikon Eclipse 600 Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse microscope. To perform the following steps you will need the following tools: Medium flat blade screw driver 3mm, 5/64 and 7/64 inch hex wrenches The hex wrenches are provided by text/html 2021-09-23T17:15:40+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_600fn?rev=1632431740&do=diff Nikon Eclipse 600FN Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse E600FN microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:39+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_800_zdrive?rev=1632431739&do=diff Nikon Eclipse 800 Microscope Motor Drive Installation This procedure steps you through the installation and alignment of the DRV-E800 motor drive onto the Nikon Eclipse 800 microscope. The following tools are required to install the ASI drive onto the Nikon Eclipse 800 microscope: text/html 2021-09-23T17:15:39+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_l200n_zdrive_install?rev=1632431739&do=diff Nikon Eclipse L200N ASI Z-Axis Motor Drive Installation This procedure steps you through the installation and alignment of the ASI’s EL200 Z-axis motor drive onto the Nikon Eclipse L200N microscope. The following tools are required for this procedure: text/html 2021-09-23T17:15:39+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_lv100_zdrive?rev=1632431739&do=diff Nikon Eclipse LV100 LV150 microscope Z-Drive Installation The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse LV100 microscope. Z-Drive Kit Should have: You will Need: * Small flat blade screw driver text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_ni_zdrive?rev=1632431738&do=diff Nikon Eclipse Ni - ASI Z-Axis Motor Drive Installation This procedure steps you through the installation and alignment of the ASI Z-axis motor drive onto the Nikon Eclipse Ni microscope. The following tools are required for this procedure: -Small Flathead Screwdriver - 1.5 text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_eclipse_ti2-a_z-drive_installation_procedure?rev=1632431738&do=diff Nikon Eclipse Ti2-A Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse Ti2-A microscope. Although not tested, the same Z-drive and instructions would work for Nikon Ti2-U as well. The procedure for installing Left or Right-hand Z-drive is identical. Only the pictures from the Left-hand Z-drive are shown below. text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_fn1_flex_zdrive_install?rev=1632431738&do=diff Nikon Eclipse FN1 Installation Procedure This is the manual for the new Flex coupler version of the Z drive. This version was introduced in December 2016. If your kit has the Flex Coupler. Then this is the correct manual. If not, then refer to the old FN1 manual. text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_fn1_zdrive_install?rev=1632431738&do=diff Nikon Eclipse FN1 Installation Procedure[Obsolete] This is the manual for the Non-Flex coupler version of the Z drive. This version was discontinued in Nov 2016. If your kit has the Flex Coupler. Then refer to the new FN1 Zdrive manual. The procedure below outlines the steps necessary to install the text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_lv300_left_hand_zdrive_install?rev=1632431738&do=diff Nikon LV-300 Left-Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon LV-300 microscope. To perform the following steps, you will need the following tools text/html 2021-09-23T17:15:38+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_microphot_fxa_zdrive_install?rev=1632431738&do=diff Nikon Microphot FXA or Microphot SA Z-Drive Installation Procedure This procedure steps you through the installation and alignment of the ASI motor drive onto the Microphot FXA or SA microscope. The following tools are required for this procedure text/html 2021-09-23T17:15:37+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_mm800_zdrive_install?rev=1632431737&do=diff Nikon MM-800 Left-Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon MM-800 microscope. To perform the following steps, you will need the following tools text/html 2021-09-23T17:15:37+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_optiphot_zdrive?rev=1632431737&do=diff Nikon Optiphot Microscope Motor Drive Installation Motor Drive Installation This procedure steps you through the installation and alignment of the ASI motor drive onto the Nikon Optiphot 2microscope. The following tools are required for this procedure: text/html 2021-09-23T17:15:37+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_optiphot2_linear_encoder_install?rev=1632431737&do=diff Nikon Optiphot 2 Linear Encoder Installation This procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Nikon Optiphot 2 microscope. The linear encoder mounts onto the microscope via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip mates with a plunger stop that is attached to the microscope’s stage carrier. The linear encoder installation has two parts: text/html 2021-09-23T17:15:37+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_optiphot200_zdrive_install?rev=1632431737&do=diff Nikon Optiphot 200 Z-Axis Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Optiphot microscope. To perform the following steps you will need the following tools text/html 2021-09-23T17:15:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_smz800_smz1000_zdrive_install?rev=1632431736&do=diff Nikon SMZ-800/1000 Z-Axis & Zoom Flex-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon SMZ-800/1000 microscope. To perform the following steps you will need the following tools text/html 2021-09-23T17:15:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_smz1500_zdrive_install?rev=1632431736&do=diff Nikon SMZ-1500 Z-Axis & Zoom Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon SMZ-1500 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:35+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_te_200_300_diaphot?rev=1632431735&do=diff Nikon Eclipse TE 200/300 or Diaphot 200/300 Microscope Z Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the left-hand focus assembly of the Nikon Eclipse TE 200/300 and Diaphot 200/300 microscopes. text/html 2021-09-23T17:15:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_te2000_left_z_install?rev=1632431736&do=diff Nikon TE2000 Left-Hand Z-Axis Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse TE2000 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_te2000_right_zdrive?rev=1632431736&do=diff Nikon TE2000 Right Hand Z-Axis Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Eclipse TE2000 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:35+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_te2000_xy_stage?rev=1632431735&do=diff Correct Installation of ASI MS-2000 Stage onto the Nikon TE2000 Microscope & Objective clearance issues When Mounting the stage the two spacers must be used as shown in figure 1. The stage should be placed on the microscope with the connectors / cables coming out on the right hand side of the microscope as shown in figure 2. If the stage is mounted incorrectly as shown in figure 3, you will have clearance problems will all objectives. The stage must be mounted as shown in figure 2. text/html 2021-09-23T17:15:33+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/nikon_um2_zdrive_install?rev=1632431733&do=diff Nikon UM-2 Right Hand Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Nikon Um-2 microscope. To perform the following steps, you will need the following tools: text/html 2021-09-23T17:15:33+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oluympus_ix70_linear_encoder_installation?rev=1632431733&do=diff Olympus IX70 Linear Encoder Installation The procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Olympus IX70 series microscopes. The linear encoder mounts to the rear of the microscope’s objective turret mechanism via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip will either mate with the rear lead screw cover on an text/html 2021-09-23T17:15:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_ax70_zdrive_installation?rev=1632431732&do=diff Olympus AX 70 Microscope Motor Drive Installation Motor Drive Installation This procedure steps you through the installation and alignment of the ASI AX 70 Z-axis motor drive onto the Olympus AX 70 microscope. The following tools are required for this procedure: text/html 2021-09-23T17:15:31+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bh2_zdrive_installation?rev=1632431731&do=diff Olympus BH-2 Microscope Motor Drive Installation Motor Drive Installation This procedure steps you through the installation and alignment of the ASI motor drive onto the Olympus BH-2 microscope. The following tools are required for this procedure: text/html 2021-09-23T17:15:31+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bx41_zdrive_installation?rev=1632431731&do=diff Olympus BX41 Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus BX41 microscope. To perform the following steps you will need the following tools: Medium flat blade screwdriver 3mm, 1/16, 5/64 and 7/64 inch hex wrenches text/html 2021-09-23T17:15:31+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bx43_zdrive_installation?rev=1632431731&do=diff Olympus BX43 Z Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus BX43 microscope. To perform the following steps you will need the following tools: Medium flat blade screwdriver 1.5 text/html 2021-09-23T17:15:31+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bx50_60_zdrive_installation?rev=1632431731&do=diff Olympus BX50\BX60 Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus BX50\BX60 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:30+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bx51_zdrive_installation?rev=1632431730&do=diff Olympus BX51 \ BX53 Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus BX51, BX53 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:31+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_bx51wi_zdrive_installation?rev=1632431731&do=diff Olympus BX51WI Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus BX51WI microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:30+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_ix70_zdrive_installation?rev=1632431730&do=diff Olympus IX70 Microscope Z-Axis Motor Drive Installation This procedure steps you through the installation and alignment of the ASI IX70 Z-axis motor drive onto the Olympus IX70 microscope. The following tools are required for this procedure: small knife or flat blade screwdriver 2.5 text/html 2021-09-23T17:15:30+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_ix73_zle_install?rev=1632431730&do=diff Olympus IX73 Z linear encoder install procedure Use a medium cross screw driver to remove the two screws that secure the cover on the right side of the microscope as shown above. Locate the linear encoder bracket, the linear encoder, and the longer screw as shown above text/html 2021-09-23T17:15:30+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_mvx10_zdrive_installation?rev=1632431730&do=diff Olympus MVX 10 Z-Axis & Zoom Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus MVX 10 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/oly_szx16_zdrive_installation?rev=1632431729&do=diff Olympus SZX16 and SZX2-FOF Z-Axis & Zoom Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus SZX2-FOF and SZX16 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:33+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/olympus_bx63_post_mount_stage_installation?rev=1632431733&do=diff Olympus BX63 Post Mount Stage Installation Please excuse the editing, this document is still being drafted. Must use non-indented top plate with MS9400. Also must add quantity of 2/ea 0.020 inch spacers to stage posts and back mount. With these spacers, focal point of slide in I-9408B insert is at 18719.04 from top travel. Complete travel range is to 20000. Large condenser can also be focused with this insert. text/html 2021-09-23T17:15:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/olympus_ix71_linear_encoder_install?rev=1632431732&do=diff Olympus IX71 Linear Encoder Installation Please excuse the editing. This document is still being drafted. 5×30mm olympus ix71 linear_encoder zdrive text/html 2021-09-23T17:15:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/olympus_ix71_zdrive_install?rev=1632431732&do=diff Olympus IX71 Microscope Z-Axis Motor Drive Installation This procedure steps you through the installation and alignment of the ASI’s IX71 Z-axis motor drive onto the Olympus IX71 microscope. NOTE: On January 2007 Olympus changed the size of the threaded end of their fine focus shaft from 3 text/html 2021-09-23T17:15:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/olympus_sz61_to_vts2000_install_note?rev=1632431732&do=diff Olympus SZ61 to VTS-2000 Installation Note Your system was configured as close to finished as possible, however because we did not have the actual microscope to work with the Z axis will have to be assembled to the microscope head. First, locate the LS-50 Linear Positioner for the Z-Axis, the mounting adapter plate, and the bag with the mounting screws, (7 total). See the adapter plate at right. text/html 2021-09-23T17:15:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/olympus_szx12_zoom_drive_installation?rev=1632431732&do=diff Olympus SZX12 Z-Axis & Zoom Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Olympus SZX 12 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/operating_temperature_limitations?rev=1632431710&do=diff **Operating Temperature Limitations** This page lists temperature limitations for various ASI Items -MS2000 LCD: -20 to 60 C text/html 2022-02-15T17:28:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/optical_and_mechanical_alignment_on_ramm?rev=1644964095&do=diff Optical and Mechanical Alignment of Epi-widefield and objective-TIRF on RAMM/MIM Correct adjustment of the illumination optics will depend upon the particular configuration of the RAMM/MIM microscope. Here we provide general guidelines for a strategy to accomplish proper alignment on systems with multiple optical paths as well as specific instructions for the particular illumination devices. As an example system we will go through the procedure for a RAMM/MIM system with both a TIRF illuminat… text/html 2022-03-22T12:53:42+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/optical_and_mechanical_alignment_transillumination?rev=1647968022&do=diff Optomechanical alignment of transillumination condenser Here we provide a strategy for alignment of our transillumination kit based upon Olympus IX2-LWUCD condenser (27 mm WD / 0.55 NA). Illuminate * Turn on the LED. * Fully open the field stop (Fig. 1); check that light is coming from the condenser. text/html 2021-09-23T17:15:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/phototrack_control_panel?rev=1632431729&do=diff Phototrack Control Panel ASI's Phototrack Control Panel is a windows .net framework based GUI to help user use ASI Phototrack system. It connects to ASI's MS2000 controller over serial port and uses serial commands to interact with MS2000. Click to Download (Save link as) Below are the descriptions and usage for the various controls on GUI text/html 2021-09-23T17:15:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/phototrack_quad_setup_procedure?rev=1632431729&do=diff Phototrack Manual Quad Setup Procedure The Settings “Quadrant Order ” (set using the LK F command) and “Cal Value” (also referred to as Gain , set using the LR X command) are critical to the proper functioning of the system. There is an Auto Calibration routine in the controller that will move the stage and figure out the values necessary for Quadrant Order and Cal Value. However if the routine fails, use this manual on how to determine these settings manually with trial and error. text/html 2021-09-23T17:15:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/phototrack_tracking_theory?rev=1632431729&do=diff Phototrack Tracking Theory Tracking laterally in XY with a quadrant photo-detector is a well established method that allows for rapid course correction without requiring digital image collection and processing. Such a tracking system images a bright emissive target onto a four-pixel quadrant detector. X and Y corrections are determined from the signal difference from X and Y detector pixel pairs. Achieving zero error difference between the adjacent pixel pairs that determine the X and Y trac… text/html 2021-09-23T17:15:40+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/phototrack?rev=1632431740&do=diff PhotoTrack Instruction Manual Installation and Calibration The PhotoTrack quadrant photomultiplier (PMT) is a C-mount device where the photo-cathode of the PMT is located at the C-mount image plane. The PMT may be attached to any microscope C-mount and be correctly positioned, however it is more common to interpose a photo-port beam splitter so that the instrument can be used with the data-recording camera. The \begin{equation}X_{ERROR} = \frac{X_+ - X_-}{X_+ + X_-}\end{equation}\begin{equ… text/html 2021-09-23T17:15:29+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/piezo_3rd_party?rev=1632431729&do=diff ASIs Drive Electronics(ADEPT) for 3rd Party Piezo Actuators ASIs Drive Electronics for Piezo Topplate (ADEPT) and actuators can drive and control 3rd party piezo stages and actuators with little or no modifications. The card is actuator agnostic and needs only a single factory calibration to be paired with an actuator. text/html 2021-09-23T17:15:28+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/piezo_install?rev=1632431728&do=diff Piezo-Z Top-Plate Installation and Handling The PZ 2000 series stage top plates are designed to provide a high resolution, and highly repeatable Z position of the microscope stage. The top plate of the stage accepts standard K size slide inserts that are available for any sample, i.e., slides, petrie dishes, multi-well plates, etc. The slide insert is moved in the Z axis via a piezo element with a range of 150 µm with nanometer accuracy (300 µm & 500 µm range are also available). By moving… text/html 2023-08-08T17:22:53+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/piezo_manual?rev=1691529773&do=diff TGADEPT & ADEPT: Drive Electronics for Piezo Top-Plate ADEPT’s Features * Closed loop operation with strain gauge feedback. * Auto strain gauge calibration on startup. * Accepts inputs from MS2000 or Tiger controller (digital) or external (analog) sources. text/html 2026-02-19T21:11:53+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/planar_correction?rev=1771553513&do=diff PLANAR CORRECTION Firmware Module This manual section applies to the PLANAR CORRECTION firmware module. With this module included in the firmware, the controller will support functions to establish a tilted focal plane by selecting three sample points. The controller can then be enabled to make a Z-axis correction such that commanded Z positions are specified with reference to the established plane. Joystick moves also follow the adjusted plane. text/html 2024-10-25T19:17:37+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/plogic_mm_plugin?rev=1729898257&do=diff ASI PLogic Control ASI PLogic Control is a Micro-Manager plugin to control the Tiger Programmable Logic Card. ASI PLogic Control comes with the latest nightly build of Micro-Manager 2.0. Requirements * Micro-Manager 2.0 * Tiger Controller * PLogic card Device Adapter The ASITiger device adapter has several modes for the text/html 2021-09-23T17:15:28+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/post_mount_setup?rev=1632431728&do=diff Camera Post Mount Kit Setup mim dispim tech_note Post Mount kit for camera support mm Parts List The kit arrives in three pre-assembled parts: * 1) universal base plus mounting screws, 2) vertical bar, and 3) safety collar not yet positioned properly * 4) cross support text/html 2025-05-15T03:28:43+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/python_code?rev=1747294123&do=diff Python Code This a hub page for Python code linked to other pages using the section tag. Example of how to embed a code section in another page in DokuWiki: { {section>:python_code#user_string&noheader&nofooter} } Remove the spaces between the curly brackets. text/html 2023-08-15T14:06:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/python?rev=1692122765&do=diff Python Python Homepage - download Python here. Many users want to interact with ASI devices through Python. There are at least 3 viable options: * Send serial commands directly using the pyserial library * Use pymmcore package to access the MMCore layer of Micro-Manager where text/html 2021-09-23T17:15:27+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/query_pzinfo_asiconsole?rev=1632431727&do=diff Using ASI Console to query PZINFO PZINFO is a very helpful command when troubleshooting piezo related issue, and ASI Console is a free Windows program provided by ASI. This tech note is a quick overview on how to use ASI Console to query the controller. Note text/html 2025-08-21T15:29:34+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/rapid_array_scanning?rev=1755804574&do=diff Rapid Array Scanning with the MS2000 Stage Introduction A common problem for automated microscopy is to quickly image an extended region that is larger than the camera field of view. This can be done by rapidly moving the stage between successive fields, stopping to snap an image at each location. Rapid stops and starts expose the inherent limitations in the mechanical system. These limitations include the inertia of the motor rotor and stage, current limitations of the motor, and how quick… text/html 2021-09-23T17:15:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/remote_refocus_microscopes?rev=1632431710&do=diff Remote Refocus Microscopes Overview In brief, remote refocus forms a 3D replica image of a sample (usually in air) with a carefully-constructed optical relay, and then a microscope looks at the replica 3D image instead of the physical one. Why go to all the trouble? Example applications are making fast Z stacks without disturbing the sample and implementing a light sheet microscope using a single objective for both illumination and detection (see Millett-Sikking and York, $n_{sample}$$EFL_{O… text/html 2021-09-23T17:15:27+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/removing_optical_tube_from_ramm?rev=1632431727&do=diff Removing the Optical Tubes from the RAMM Tools Needed: * 2mm socket driver and * 2mm Allen wrench STEP 1: Locate the tube on the RAMM. STEP 2: Remove the top two metric set screws with the 2mm Allen wrench. STEP 3: Remove the bottom two set screws with the 2mm socket driver. text/html 2021-09-23T17:15:27+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/removing_red_shipping_bracket?rev=1632431727&do=diff TN150: Removing the Red Shipping Bracket from the RAMM Tools Needed: * 5mm and 6mm driver or Allen wrench STEP 1: Locate the Red shipping bracket on the RAMM. It is attached between the Arch and the Linear Positioner, LS-50, and should be obvious. text/html 2021-09-23T17:15:12+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/removing_rf_mod_cap?rev=1632431712&do=diff Removing the RF(capacitor) modification Between mid 2018 to mid 2019 a small capacitor was added to MS2000, RM2000 controllers to reduce RF emissions. When this capacitor fails it grounds the oscillator preventing the microcontroller from running. The controller doesn't respond to serial/usb input, joystick, knob and the LCD is blank or full of bars. text/html 2021-09-23T17:15:11+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/reorient_filter_wheel_connector_housing?rev=1632431711&do=diff tech_note fw1000 How to Reorient a FW-1000 Connector. The default orientation of a Filter Wheel connector is to the left if you have the motor tower towards the ground, and the filter port facing away from you. It is simple to reorient this connector housing, but you should be cautious. If the wires are crushed, the unit will malfunction. If the motor shaft rubs through the insulation on the wires, the unit will malfunction. text/html 2021-09-23T17:15:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/repairing_fw1000_filter_wheel_encoder_head?rev=1632431726&do=diff Repairing the FW-1000-4-40mm Filter Wheel Encoder Head Be sure the power is off and the Wheel is removed from the controller. You will need a Phillips screwdriver, a 7/64” hex ball driver, a 3/32” hex ball driver, a 4mm hex ball driver, two small Allen wrenches and the replacement encoder. text/html 2021-09-23T17:15:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/repeatability_performance_of_asi_stage?rev=1632431726&do=diff Repeatability Performance of ASI Stages Microscope stages are often used in demanding applications where the stage’s ability to return precisely to a given location is a requirement. ASI stages may be equipped with either standard rotary shaft encoders on the drive motors, or high precision linear encoders on the stage plates themselves. The stage controller has an anti-backlash routine which can be used to advantage for precision movement. This technical note will explore how these factors … text/html 2021-09-23T17:15:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/replacing_5volt_power_supply_tiger?rev=1632431726&do=diff Replacing the 5Volt power supply on TG-8 and TG-16 controllers The following technical notes shows how to replace the +5Volts and -5Volts power supplies used in TG-8 and TG-16 controllers. The power supply is mounted on the back panel of the controllers where the AC socket is located. text/html 2021-09-23T17:15:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/replacing_filter_on_mippi3?rev=1632431726&do=diff Replacing the filter on the MPPI-3 (Be sure the power and the pressure is off to the system. You will need a replacement filter and also a Flat Head and Phillips Screwdriver and a ½” Wrench or adjustable Crescent Wrench to remove the cover, filter cover and filter). text/html 2021-09-23T17:15:25+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/replacing_leds?rev=1632431725&do=diff Replacing LED boards in ASIs LED Illuminator(MIM-LED-LAMP) This Technical note walks users thru on how to replace the LED board(or LED) in ASIs LEDs illuminator (MIM-LED-LAMP or MIM-LED-LAMP-NR assembly) Tools Needed * 1.5mm Hex key or allen wrench text/html 2021-09-23T17:15:25+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/resetting_the_pulse_duration_knob_on_mippi?rev=1632431725&do=diff Resetting the Pulse Duration knob on MPPI Be sure the power and the pressure is off to the system. You will also need a .9 mm Allen wrench to remove the knob and perhaps a ½ wrench or pliers to tighten the potentiometer. Step 1: On a clean work table lay out the MPPI and the Allen wrench. Locate the Pulse Duration knob and rotate it counter clockwise back to the zero position. The lock lever should be in the upright position as seen in figure 1 to allow the smooth rotation of the knob. When yo… text/html 2023-08-24T20:50:55+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ring_buffer?rev=1692924655&do=diff RING BUFFER MODULE Often there is a need to mark several positions to later revisit. The RING BUFFER module enables a buffer with up to 50 locations that the user can load with position information and then visit sequentially. Many firmware builds can allow 250 positions; contact text/html 2025-06-24T13:41:46+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ringtirf?rev=1750786906&do=diff ASI Ring TIRF Control The ASI Ring TIRF Control plugin is used to control a Ring TIRF microscope using ASI hardware. This plugin comes with the latest nightly build of Micro-Manager 2.0 as of November 2022. Requirements * Micro-Manager 2.0 * Tiger Controller * text/html 2021-09-23T17:15:24+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/safety_instructions?rev=1632431724&do=diff Safety Instructions Please read these instructions carefully; they include important information about the installation, usage and maintenance of this product. * Always make sure that you are connecting to proper voltage, and that the line voltage you are connecting to is not higher then the rating of the installed fuse. text/html 2021-09-23T17:15:24+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/safety_notices?rev=1632431724&do=diff Safety Notices This user documentation contains safety notices (Warning, Caution, Notice, etc) concerning the safe use of the product. See definitions below. Warning: Indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood. text/html 2026-03-24T21:25:27+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/scan_module?rev=1774401927&do=diff SCAN MODULE Sync and Scanning Addendum Raster and serpentine scanning has been made simple and flexible with some special firmware and hardware options added to ASI's standard controller. Raster scanning is often used in conjunction with other equipment which collects data during the scan, the goal being data generation that can be mapped accurately in the two spatial scan dimensions. To facilitate this, the \begin{equation}S <= \frac{4000 * EncoderDivide}{counts/mm}\end{equation} text/html 2024-11-26T18:31:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/sequencer?rev=1732663882&do=diff SEQUENCER MODULE WARNING: The Sequencer module has been deprecated since ~2015. The Tiger Programmable Logic Card can replace most of the functionality. Overview Old documentation that you can download in PDF form: ASI MS2000 SEQUENCER MODULE The SEQUENCER module consists of several hardware signal outputs, both TTL signals (TTL#) and Analog Voltage Outputs (AVO#). Incremental stage motion can be directly controlled in a sequence as well (STG#). A TTL trigger INPUT and various RS232 se… text/html 2025-05-15T19:49:52+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/serial_out?rev=1747352992&do=diff Serial Output The SERIAL OUTPUT firmware module uses a separate serial port to output data. This firmware module can be used with the TTL T=51 report mode for MS-2000. ms2000 text/html 2025-04-09T18:33:00+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/serial_position_report?rev=1744237980&do=diff Serial Position Report This feature reports the time and positions of the enabled axes, the reports can be enabled with a serial command or TTL input. The TTL X=5 input mode enables/disables position reporting. RT M+ starts the reports, RT M- stops the reports. RT X=milliseconds sets the interval between reports, the default is 200 text/html 2025-03-17T15:32:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/servolock_ttl?rev=1742239956&do=diff SERVOLOCK_TTL The SERVOLOCK_TTL firmware module allows the user to control the stage position bi-directionally using TTL pulses. When placed in the SERVOLOCK_TTL state, motors are kept on with servo loop active and the target position is adjusted every time a TTL pulse is received. The duration of each TTL pulse determines whether a positive or negative adjustment is applied. The adjustment magnitude is the same as the last relative move and can be changed via serial command even when the text/html 2021-09-23T17:16:01+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/sidebar?rev=1632431761&do=diff Back to main ASI website Products Tags text/html 2026-02-12T19:56:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/singleaxis?rev=1770944207&do=diff Single-axis functions The single-axis firmware module lets the controller move the actuators in patterns including sawtooth, triangle, and square waves. This module is intended to run off a 4Khz internal clock, though provision has been made to accept an external clock on the TTL input (only lightly tested). They can also be configured to output TTL pulses upon completion of each cycle. For both these TTL functions, the corresponding physical connectors need to be in place. text/html 2025-09-15T15:57:58+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/slave_f_to_z_module?rev=1757966278&do=diff SLAVE_F_TO_Z Module Operation (FTP firmware) Controllers with the SLAVE_F_TO_Z module facilitates use of a master/slave set of motorized axes. When the master axis is moved or adjusted then the slave axis is correspondingly changed automatically. The user should almost always interact with the master axis so that the two axes remain synchronized. During setup it may be helpful to interact with the slave axis to get them mechanically aligned. text/html 2021-09-23T17:15:23+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/slow_speed_considerations?rev=1632431723&do=diff Slow Speed Considerations Some users find it necessary to run stages at very slow and steady scanning speeds. For a mechanical stage system, ASI stages and controllers manage to do a pretty good job of this, but there are limitations. This document helps to clarify what has been observed at very slow speeds and what can be expected of the stage in various configurations.\begin{equation} D = \frac{screw-pitch}{gearhead-reduction} \\ D = \frac{1.59}{141} = 11.3 μm/rev \end{equation}\begin{e… text/html 2026-04-10T15:24:02+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/software?rev=1775849042&do=diff Software This page contains manuals and download links for software developed by ASI. Firmware Updates: ASI Console - update firmware and interact with MS-2000, RM-2000, and FW-1000 controllers. Tiger Controller Console - update firmware and interact with TG-1000 controller. Micro-Manager Plugins: text/html 2021-09-23T17:15:23+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/solenoid_lock_insert?rev=1632431723&do=diff Solenoid Lock Insert (I-3020-MLLAC) I-3020-MLLAC is a solenoid powered insert that is able to secure a Multi-well plate in place. The insert is driven with ASI MS2000 or TG-1000 controller. The insert is operated with the SECURE command ms2000 tiger manual solenoid_lock text/html 2025-03-18T19:30:06+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/speed_truth_module?rev=1742340606&do=diff Speed Truth Firmware Modules Speed Truth Module The SPEED_TRUTH firmware module is only used on the MS-2000 controller. When SPEED_TRUTH is included in the firmware build, the SPEED, AC, and INFO commands will output the actual values for speed and acceleration rather than the requested values. text/html 2021-09-23T17:15:09+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/stablestage?rev=1632431709&do=diff ASI Stable Stage The ASI Stable Stage plugin can measure and correct thermal drift. Requirements: Micro-Manager 2.0 Optional: CRISP or Autofocus3 (Note: Autofocus3 integration coming soon) Send an email to brandon@asiimaging.com if you're interested in the plugin. text/html 2025-03-17T20:30:25+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/stage_accuracy_settling_time_for_asi_stage?rev=1742257825&do=diff Stage Accuracy and Settling Time for ASI Stages The reproducibility and accuracy of a servo stage usually hinges on its behavior while seeking its final position. The stage may be required to come to rest within one to a few encoder counts of the desired location. Mechanical effects including vibration, inertia, and friction can frustrate efforts to land precisely. Careful tuning of the servo feedback parameters can help the system to quickly settle on target, but it usually takes longer to … text/html 2022-10-12T14:21:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/stage_packaging?rev=1665598870&do=diff Packaging an XY Stage for Shipment Step 1: Begin by inserting the shipping bolt in the stage. If you no longer have a shipping bolt or your stage is not fitted to use one, secure the stage with either zip ties or tape as available so that the plates cannot move during shipping. text/html 2023-07-13T16:51:20+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/start?rev=1689281480&do=diff Welcome to the online documentation for Applied Scientific Instrumentation. Legacy documentation is available in PDF form, but is no longer being updated. The wiki contents are more comprehensive, searchable, and cross-referenced. To download a collection of pages for offline reference use the text/html 2021-09-23T17:15:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/swap_lids_ms2000?rev=1632431722&do=diff Swapping Lids on the MS-2000 (WARNING: You should be familiar with the MS/MFC-2000 manual before attempting the lid swapping) STEP 1: Turn off the power and remove all cables attached to the controller. STEP 2: Remove the six case screws located on the back top and bottom front of the controller, (to avoid stripping screws when removing bottom screws, apply pressure to keep the top and bottom covers together while removing/inserting screws). text/html 2021-09-23T17:15:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/synchronized_zaxis_focus_sweeps?rev=1632431722&do=diff Synchronized Z-axis Focus Sweeps Focus sweeps provide a simple way to integrate many focal planes in a single image. It is possible to generate accurate position and exposure control using some specialized scanning firmware and hardware encoder comparisons with the MS2000WK controller and an \begin{equation} Speed = \frac{Focal Range}{Exposure Time} \\ Speed = \frac{0.010 mm}{0.200 sec} = 0.05 mm/sec \end{equation} text/html 2026-01-28T21:50:35+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/synchronous_encoder_reporting?rev=1769655035&do=diff Synchronous Encoder Reporting TTL Report Module This section of the manual applies to the TTL_REPORT_INT firmware module. Including this module in the firmware allows for precisely synchronized encoder position reporting. The user's hardware supplies a TTL trigger to the MS-2000 that causes a processor interrupt which performs an encoder-read operation on all of the controller’s motorized axes. Any firmware build that includes this module is not capable of any other TTL input modes and will on… text/html 2021-09-23T17:15:11+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/targets?rev=1632431711&do=diff Alignment Targets Print these, then cut them out on the outer edge to have convenient targets for safe and quick beam alignment. [A round printable target for aligning C-Mounts and tubes] [A square printable target for aligning 60mm cubes and brackets] target sight reticle alignment text/html 2021-09-23T17:15:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tech_note_changing_piezo_drive_card_fast_slow_compensation?rev=1632431722&do=diff Changing Piezo Drive Card's Fast Slow Compensation Dynamics of an ASI Piezo Top-plate and Piezo Objective Mover (FPOMs) vary depending on piezo travel and sample weight they are subjected to. Heavier payload (500grams and above) and longer piezo travel range(300um and above) tend to make the stage more unstable or prone to ringing. These piezo stages need a slower error compensator on the piezo drive card to work optimally. text/html 2021-09-23T17:15:22+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tech_note_crisp_error_calculation?rev=1632431722&do=diff CRISP Error Calculation Algorithm At focus ideally what we want: \begin{multline} \\ PD_1 = 75 \\ PD_2 = 75 \\ SUM = \frac{ PD_1 + PD_2}{2} = 75\\ OFFSET = 0 \\ ERR = (PD_1-PD_2) - OFFSET = 0\\ \end{multline} Returned light is distributed evenly between the two halves of the detector and is hitting the center of the detector pair. Small changes in intensity of the returned light will not affect the ERR. If PD1 and PD2 suddenly see only 70, still ERR = 0 so no ERR signal app… text/html 2025-03-20T17:33:34+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tech_note_rs232_comm?rev=1742506414&do=diff RS-232 Communication All ASI Controllers (TG-1000/Tiger, MS-2000 and RM-2000) have an RS-232 serial link. While the controller can be used in stand-alone mode completely separate from a computer, having a connection to send serial commands is required to utilize all of the controller’s abilities. There is a separate page with an text/html 2026-01-07T17:23:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tg1000_info?rev=1767824585&do=diff TG-1000 General Information Electrical Characteristics AC Input: 90-264 VAC, 3.3 A (peak), 50/60 Hz AC In-rush Current (Cold Start): 77 A (maximum), at 240 VAC Fuse Replacement: 2.0 A Fast Blow 250 VAC (Spare fuse located in Power Jack on Back Panel) Mains supply voltage fluctuations not to exceed 10% of the nominal voltage Indoor use only text/html 2026-02-03T17:33:53+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tggalvo?rev=1770158033&do=diff TGDAC4 / TGGALVO (4 Channel DAC card) CAUTION! On few early prototypes, at startup the card outputs will swing to -10Volts briefly. This may damage instruments that aren't designed to handle negative input. The TGDAC4 is a versatile card capable of performing many different roles. text/html 2023-04-07T03:41:06+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tgled?rev=1680853266&do=diff TGLED and LLED-xxx-yz User Guide [ ] ASI LED Overview ASI LED illuminators are designated by the part number LLEDxxx-yz. * xxx indicates the approximate central wavelength in nanometers, or WHT for white. The LED wavelengths listed below can be used with any of the drive electronic types. text/html 2023-03-26T05:26:06+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tgpmt?rev=1679822766&do=diff TGPMT / PMT-200 The PMT-200 (also referred as TGPMT) card is part of the TG-1000 Tiger Controller that lets users power and interface with two Photo Multiplier Tubes (PMT). Features * High Sensitivity * Controls up to 2 PMTs * Manual or Programmable PMT Gain text/html 2025-09-22T14:03:50+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_control_panel?rev=1758564230&do=diff Tiger Control Panel Tiger Control Panel (TCP) is a Windows program to communicate with the TG-1000 controller. It provides a serial terminal to send commands to the controller. t also comes with many utilities on the side panel and is able to report the position of all axes, axis limit status, toggle buttons, etc. text/html 2025-02-25T13:41:05+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_ms2k_difference?rev=1740508865&do=diff TG-1000 and MS-2000's Instruction Set Differences This section is intended to quickly bring the users up to speed on the major differences in the serial commands between TG-1000 and MS-2000 controller. Where possible the TG-1000 commands were kept close to the MS-2000 controller commands. The most common commands are identical because they are Axis-Specific commands. A special text/html 2021-09-23T17:15:20+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_not_booting_troubleshoot?rev=1632431720&do=diff TG-1000 Controller Troubleshoot This page has simple steps a user can try to fix issues with a TG-1000. Controller does not BOOT/Start and 5V LED is OFF In case the controller isn't starting up, and the 5V and status LED on each card is OFF. Like for the controller shown below. text/html 2021-09-23T17:15:20+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_phototargeting?rev=1632431720&do=diff TG-1000 Phototargeting The ASI scanner can be used as a photo-targeting device, for example for FRAP, photo-uncaging, optogenetic stimulation, etc. A special firmware module MM_TARGET for the micro-mirror card exists for this application. The vast majority of commands are the same, but differences and special functions are outlined here. The text/html 2021-12-20T16:55:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_piezo_dac?rev=1640037336&do=diff Tiger Piezo DAC Card ASIs Tiger Piezo DAC card enables the tiger system to control two piezo controller via two 0-10V signals. The Tiger system can support multiple Piezo DAC cards, these card can then be controlled thru RS-232 serial commands or thru Knobs. text/html 2026-03-16T14:03:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_programmable_logic_card?rev=1773684239&do=diff Tiger Programmable Logic Card (TGPLC) ASI's Programmable Logic Card (PLC) is a field-programmable card for digital logic, sort of a mini-FPGA inside the Tiger controller. It acts as a single axis device (by default, axis E) and occupies one Tiger address (e.g. 36 in a diSPIM TG-16). Its original use is to enable multi-laser control for diSPIM, but it is designed to be very flexible and can be used for other tasks involving digital signals. text/html 2023-03-26T05:53:26+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_tunable_lens_card?rev=1679824406&do=diff Tiger Tunable Lens Card (TGTLC) Description The TGTLC is a Tiger card that can power 2 Electrically Tunable Lenses (ETL) such asOptoTune's EL-10-30. ETLs are polymer lenses whose curvature and thus focal length can be changed by applying current. They are available with diverse cover glass coating and optional offset lens. $f_{eff} = 1/(1/f_{ETL} + 1/f_{offset})$$D_{eff} = D_{ETL} + D_{offset}$\begin{equation}\delta_{focus} = \frac{-1}{M_{det}^2}*\frac{n*f_r^2}{f_{ETL}}\end{equation}\begin{e… text/html 2025-05-22T15:44:59+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tiger_w_commands?rev=1747943099&do=diff Wishbone/Low Level Commands for TG1000 The TG-1000 controller can be interfaced through High Level commands(HL cmds) and W Commands(W cmds). HL cmds use ASCII characters, human readable, very verbose, easy to construct. W cmds are binary and ideal for hardware to hardware communication. In fact all internal communication between Tiger Comm card and Device cards are done in W cmds. No special operation is required to switch from HL cmds to W cmds, Tiger Comm automatically detects and processes t… text/html 2023-04-02T07:29:10+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tirf_alignment_old?rev=1680434950&do=diff Old TIRF Assembly and Alignment Procedures This is the legacy TIRF assembly and alignment procedures documentation, please see the new documentation for the current procedures. ASIAligning the TIRF Direct exposure to laser can lead to an eye injury. Please follow all safety precautions. text/html 2023-04-02T07:19:32+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tirf_alignment?rev=1680434372&do=diff TIRF Install and Alignment Direct exposure to laser light can lead to an eye injury. During alignment, the laser beam may be unpredictable. Use low laser power, physical beam-stops and wear protective eye-wear. When aligning a TIRF (Total Internal Reflection Fluorescence microscopy) system, one must be considerate of the tilt, and focus of the excitation light. The goal is to have the excitation beam be slightly off-center but perpendicular to the back focal plane of the objective. The TIRF … text/html 2024-03-05T19:07:43+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tirf_overview?rev=1709683663&do=diff TIRF Microscopy Overview TIRF Microscopy uses the phenomenon of Total Internal Reflectance (TIR) to perform Fluorescence (F) illumination in a very thin layer barely into the sample side of a glass/sample interface. It has proved useful for studying surface regions of cells with minimal background from deeper regions. $\delta$$EFL_{obj}^2/\delta$$\delta<<EFL_{obj}$$FOV_{radius} * \delta / EFL_{obj}^2$$\delta$ text/html 2025-07-16T18:11:52+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tn_fw1000_move_speeds?rev=1752703912&do=diff FW-1000 move times There are SV settings that you can give the FW-1000 controller to adjust the speed and aggressiveness of the Filter Wheel. Click on the image below for a larger view. The default setting is SV7 which is ~50ms for adjacent moves. text/html 2021-09-23T17:15:18+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/troubleshoot_z_axis?rev=1632431718&do=diff Trouble Shooting ASI Z-axis drives Configuration ASI Z-axis drives are precision closed –loop D.C. servo motor systems that employ the following components: 1. A D.C. servomotor with a gear head. 2. An electromechanical clutch that allows the drive to be engaged & disengaged. text/html 2021-09-23T17:15:18+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/tuning_40_tpi_stage?rev=1632431718&do=diff Tuning Your 40 TPI Lead screw Stage for Small Moves Dear User, your stage has been optimized for small move sizes (100nm-10nm). Various physical and firmware settings were modified to accomplish this. To achieve 10nm moves we found the following settings helped. text/html 2021-09-23T17:15:18+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/two_axis_control_and_knob?rev=1632431718&do=diff Two-axis Control with Command Knob More than one axis can be controlled with the command knob on the controller. Commonly, this is because in addition to a mechanical Z-axis drive, there may also be a Piezo-Z system or a Zoom axis configured in the controller. text/html 2025-10-09T16:43:41+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/ultimate_resolution_of_microscopes?rev=1760042621&do=diff Resolution Limits of Optical Microscopes and Related Requirements for Mechanical Stages Optical Resolution The wave nature of light imposes fundamental limitations on the resolution of an optical system. For a self-luminous body, as in fluorescence microscopy, the resolving ability is commonly defined using the Rayleigh criterion: a point source of light can barely be resolved from a neighboring point source when spaced by the Airy disk radius. It can be shown that this distance $d_{xy}$\beg… text/html 2023-12-04T19:44:28+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/using_zstack_array_module?rev=1701737068&do=diff Z-Stacks Using the Array Module Overview This document gives a short summary of using the ASI command set to achieve Z-stacks using the ARRAY firmware module; refer to the programming manual for full explanations of these commands. You can check to see if the firmware has the ARRAY functionality using the BU X command, if one of the lines in the reply is text/html 2023-01-25T13:57:36+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/video_autofocus?rev=1674673056&do=diff Video AUTO-FOCUS The ASI auto-focus system uses an electronic circuit to detect the high spatial frequency information present in a video signal coming from an analog camera, and converts it to a focus value for each frame – the better the focus, the larger the focus value. Depending on the sample and the type and power of the objective, the conditions to achieve the best focus value may change. To compensate, the system incorporates an auto-calibration routine that automatically sets various… text/html 2023-10-25T13:29:02+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/warranty?rev=1698254942&do=diff Five-Year Warranty for Routine Laboratory Use Applied Scientific Instrumentation, Inc., hereafter referred to as ASI, guarantees its products for routine laboratory use against all defects in materials and workmanship to the original purchaser for five (5) years text/html 2021-09-23T17:15:17+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/xystage_limits?rev=1632431717&do=diff Setting Limit Switches on Various XY Stages There are two sets of limit switches on nearly all XY stages. The two on the top of the stage as shown figures 1a & 1b below are used to limit the range of motion in the Y-axis. The two shown figure 2 a & 2b below are used to limit the range of motion in the X-axis, and these are located on the bottom of the stage. The X-axis limit adjustment setscrews can be accessed by moving the Y-axis towards the front of the microscope. You will need an Allen wr… text/html 2021-09-23T17:15:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_av35_zdrive?rev=1632431716&do=diff Zeiss AXIOVERT-35 Z Drive Installation Procedure (left side drive) The instruction steps below describe how to install the ASI motor drive on to the Zeiss Axiovert-35 Microscope. The procedure has three parts: 1. Removing the plastic bottom cover from the underside of the microscope and installing in its place the motor drive base plate. text/html 2021-09-23T17:15:17+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_av100_av135_zdrive?rev=1632431717&do=diff Zeiss Axiovert 100-135 Series Microscope Z-Drive install Procedure The instruction steps below describe how to install the ASI motor drive onto the Zeiss Axiovert 100 Series Microscope (left side drive). The procedure has three parts: * Preparing the microscope to be inverted and installing the text/html 2021-09-23T17:15:17+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_av200_zdrive_install?rev=1632431717&do=diff Zeiss Axiovert 200 (AV200) Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Axiovert 200 microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioexaminer_ftp_install?rev=1632431716&do=diff Zeiss AxioExaminer FTP-2100 installation Remove the top cover as shown & loosen the six screws noted with a 5 mm Allen wrench as shown. Remove top of scope as shown & then used T25 wrench to remove four screws noted that secure factory spacer text/html 2021-09-23T17:15:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioimager_zdrive?rev=1632431716&do=diff Zeiss AxioImager Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss AxioImager microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axiolab_zdrive?rev=1632431716&do=diff Zeiss Axiolab Microscope Z Drive Installation Procedure Motor Drive Installtion The procedure below outlines the steps necessary to install the ASI Z-Axis Drive onto the Zeiss AxioLab microscope. To perform the following steps you will need a large, medium and small slotted screw driver, a small phillips screw driver, a 14mm deep socket wrench and the following hex wrenches: 7/64”, .050”, 3mm and 1.5mm. The socket wrench and hex wrenches are provided by text/html 2021-09-23T17:15:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioplan_axioskop_zdrive?rev=1632431715&do=diff Zeiss Axioplan I, Axioskop I, Axioskop FS Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Axioplan I/AxioskopFS /AxioskopFS microscope. To perform these steps you will need the following tools: text/html 2021-09-23T17:15:16+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioplan2_zdrive_install?rev=1632431716&do=diff Zeiss AxioPlan II Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss AxioPlan II microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioskop_2_zdrive?rev=1632431715&do=diff Zeiss Axioskop II Z-Axis Focus Controller Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Axioskop II microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioskop_linear_encoder_install?rev=1632431715&do=diff Zeiss Axioskop linear encoder Installation The procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Zeiss Axioskop microscope. The linear encoder mounts to the rear of the microscope via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip mates with a plunger stop that is attached to the microscopes stage carrier. The linear encoder installation has three parts: text/html 2021-09-23T17:15:15+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axioskop_xystage?rev=1632431715&do=diff Installing the Adapter Ring onto an Axioskop Microscope Place the adapter ring on your microscope. The deepest part of the notches face down toward the bottom of the microscope. Secure the ring to the microscope using the 4m x 8m allen head screws and washers supplied. An allen wrench is supplied for this. When the ring is secure, place the stage on top so that the two tabs in the rear of the stage fit into the notches on the adapter ring. Secure the stage onto the ring by tightening the thumb… text/html 2021-09-23T17:15:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axiostar_plus_zdrive_install?rev=1632431714&do=diff Zeiss Axiostar Plus Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Axiostar Plus microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axiovert_200_linear_encoder_install?rev=1632431714&do=diff Zeiss Axiovert 200 Linear Encoder Installation The procedure outlines the steps necessary to install and align the ASI Heidenhain linear encoder onto the Zeiss Axiovert 200 series microscopes. The linear encoder mounts to the rear of the microscope’s objective turret mechanism via an encoder clamp. The linear encoder has a plunger that depresses into the encoder as the focusing position is moved. The plunger tip will either mate with a plunger stop on an text/html 2021-09-23T17:15:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_axiovert_stage_install?rev=1632431714&do=diff Zeiss Axiovert Observer stage mounting screw locations Location of three 4 x 12 mm screws that secure stage to Axiovert /AxioObserver. There are two in the front & one in rear. Use 3mm Allen wrench to remove screws & then remove stage. zeiss xystage axiovert text/html 2021-09-23T17:15:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_standard_16_zdrive?rev=1632431714&do=diff Zeiss Standard 16 Motor Drive Installation The procedure below outlines the steps necessary to install the ASI Z-Axis Drive onto the Zeiss Standard 16 microscope. Pleas note that the terms left and right of the microscope refer to viewing the microscope from the front. text/html 2021-09-23T17:15:14+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_stemi_2000c_zdrive_install?rev=1632431714&do=diff Zeiss Stemi 2000C Z-Axis & Zoom Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Stemi 2000C microscope. To perform the following steps you will need the following tools: text/html 2021-09-23T17:15:13+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_universal_zdrive?rev=1632431713&do=diff Zeiss Universal Z-Drive Installation Procedure The procedure below outlines the steps necessary to install the ASI Microscope Focus Controller Drive onto the Zeiss Universal microscope. Please note that the terms left and right of the microscope refer to viewing the microscope from the front. text/html 2021-09-23T17:15:13+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zeiss_xystage_warning?rev=1632431713&do=diff Warning for Zeiss XY Stage Installing screws incorrectly Will Damage Stage & Void your Warranty. Follow These Instructions mmmm If you do not have the correct screws or your stage carrier is different than the one shown here, please contact ASI before proceeding. text/html 2025-08-20T16:31:47+00:00 Anonymous (anonymous@undisclosed.example.com) http://www.asiimaging.com/docs/zflock_module?rev=1755721907&do=diff ZF LOCK (FTP firmware) module Operation Controllers with the ZFLOCK module let the user slave or un-slave an axes to another at runtime. When slaved, slave axis will mirror all moves and events that happen on master axis. This mode is enabled and disabled thru the serial command