======= ASI Stable Stage ======= The ''ASI Stable Stage'' plugin can measure and correct thermal drift. Requirements: ''Micro-Manager 2.0'' Optional: ''CRISP'' or ''Autofocus3'' (Note: ''Autofocus3'' integration coming soon) Send an email to ''brandon@asiimaging.com'' if you're interested in the plugin. ==== Controls ==== {{:stablestage_maintab.png?direct|}} **Main Tab:** **1) Run Forever:** Check this box if you would like to run the test indefinitely in ''Drift Measurement'' or ''Stage Stabilization'' mode. **2) Test Length:** If you're not running the test indefinitely, then you can specify the number of minutes you would like the test to run. **3) Drift Error:** The amount of drift in microns required for ''Stage Stabilization'' to attempt to correct the XY stage position. **4) Max Move:** The maximum amount that ''Stage Stabilization'' will move the stage in microns. **5) Phase Correlation:** Which phase correlation algorithm to use for ''Stage Stabilization''. **6) Autofocus Device:** Which autofocus device to use for ''Stage Stabilization''. Select ''None'' if no device available. **7) XY Moves:** Invert the computed shift values in either the X or Y axes. **8) XY Gain:** A multiplier to adjust the computed shift value. {{:stablestage_cameratab.png?direct|}} **Camera Tab:** **1) Show Images:** Check this box to show the phase correlation comparison images while running the test. **2) Keep Shutter Open:** Keep the cameras shutter open during the test. **3) Capture Delay:** The number of milliseconds to delay between each phase correlation comparison test. **4) Downsample Factor:** The amount to downscale the camera images, this can increase performance significantly. **5) Apply Gaussian Blur:** Apply a blur filter to the captured images, this setting is recommended. **6) Blur Amount:** The amount of blur to apply to the images, the standard deviation of the Gaussian filter. **7) Use Background Subtraction:** Check this box if you want to use background subtraction. **8) Snap Background Image:** Snaps an image to use as the background subtraction image. **9) View Background Image:** Opens a frame to view the captured background subtraction image. {{:stablestage_datatab.png?direct|}} **Data Tab:** **1) Save Directory:** The directory to save the images into. **2) File Name:** The file name prefix to save the logging data. **3) Logging:** Check these boxes to log information about XY moves and Z moves. **4) Save Images:** Check this box to save images into the save directory. **5) Save Type:** The criteria for saving images, the spinners below control the options for save types. **6) Save Every N Images:** This is always save the first image, and then every N images afterward. **7) Move Threshold:** Save the test image a move command above a certain threshold is sent to the controller. **8) Correlation Threshold:** Save the test image if the correlation coefficient of the comparison is below this threshold. **9) Limit Save Data:** The maximum amount of image data to save in a test run in gigabytes. {{tag> software auto_track}}