Recent cleared tissue light sheet pre-prints with ASI contributions

Two pre-prints with light sheet microscopes with ASI contributions have been recently submitted to bioRxiv.  Eventually these should appear in traditional journals, but I applaud the authors’ decision to make them available on bioRxiv sooner (I’m a big fan of pre-prints for multiple reasons).

First, a pre-print from the Allen Institute for Brain Science describing mFISH in cleared mouse brain imaged using light sheet [fn1].  The system uses an ASI iSPIM setup, ASI’s NA 0.4 multi-immersion objectives (developed with Special Optics), and the ASI diSPIM plugin in Micro-Manager to perform image acquisition with stage scanning.  The focus of the paper is biology which I don’t pretend to fully understand, but it’s been fun helping Rusty and others on the instrumentation side of things.

Second, a pre-prent from the University of Washington describing the latest iteration of their open-top light-sheet microscope for high-throughput imaging [fn2].  The system uses ASI stages (the XYZ stage ASI calls the “FTP-2000“) and the same NA 0.4 multi-immersion objective.  I’m glad the analysis I did years ago on the maximum achievable imaging depth as a function of objective NA and WD is now published as part of Supplementary Figure 22.  It’s been a enjoyable helping Adam and others on the hardware and it was a pleasant surprise that he wanted me to be a co-author.

[fn1] Multimodal cell type correspondence by intersectional mFISH in intact tissues, DOI: 10.1101/525451

[fn2] Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues, DOI: 10.1101/548107

ASI’s new compact 3D/4D Stage

ASI’s new compact 3D/4D stage is a precise motorized motion control system designed to move samples around fixed optics. It incorporates three ASI linear stages and an optional motorized rotating stage employed for a theta axis. The linear stages comprising the XYZ elements offer travel options of 25, 50, 100, or 200 mm and each axis can be chosen separately. The linear stages derive smooth and accurate motion from closed-loop DC servomotors, crossed-roller bearings, high-precision lead screws, and high-resolution encoders for positioning feedback. Like other ASI stages, these can move uniformly at extremely slow speeds for in-motion acquisition. All the stages offer various speed/accuracy options for a more customized 3D/4D system. The stage elements are rigidly attached together, and usually mounted to a breadboard via an adapter plate.

ASI offers a variety of controllers that are compatible with this stage, including the MS-2000 and the modular Tiger controller. All provide automatic backlash correction, and can communicate with a host computer by RS-232 or USB connection.

Know more about it.